anti-Dengue virus NS4B antibody [SQab19172]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [SQab19172] recognizes Dengue virus NS4B
  • 反应物种: DEN
  • 应用: ELISA, ICC/IF, WB
  • 特异性: This antibody recognizes DENV2 and DENV3 Membrane protein
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: SQab19172
  • 同位型: IgG1
  • 靶点名称: Dengue virus NS4B
  • Brand:
CAT.NO. : ARG66683
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Product Details
概述
产品描述Mouse Monoclonal antibody [SQab19172] recognizes Dengue virus NS4B
反应物种DEN
应用ELISA, ICC/IF, WB
特异性This antibody recognizes DENV2 and DENV3 Membrane protein
宿主Mouse
克隆Monoclonal
克隆号SQab19172
同位型IgG1
靶点名称Dengue virus NS4B
抗原物种Dengue virus
抗原Dengue virus NS4B.
偶联标记Un-conjugated
应用说明
应用建议
应用推荐稀释比
ELISAAssay-dependent
ICC/IF1:1000
WB0.5 µg/ml
应用说明*Western: DENV3 not workable; ELISA: DENV3 not workable.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液PBS (pH 7.4) and 0.01% Thimerosal.
抗菌剂0.01% Thimerosal
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
检测图片 (4)
  • ARG66683 anti-Dengue virus NS4B antibody [SQab19172] ICC/IF image

    Immunofluorescence: Mock infected Huh7 cells (upper panel) and DENV infected Huh7 cells (lower panel). Cells were fixed with 4% paraformaldehyde for 30 mins, permeabilized by 0.5% Triton-X 100 for 10 mins and blocked for 1 hour. Cells were stained with ARG66683 anti-Dengue virus NS4B antibody [SQab19172] (green) at 1:1000 dilution, overnight. Nuclear staining (blue).

  • ARG66683 anti-Dengue virus NS4B antibody [SQab19172] WB image

    Western blot: 10 µg of Vero, ZIKV-infected Vero and DENV2-infected Vero cells (left to right). Cell lysates were stained with ARG66683 anti-Dengue virus NS4B antibody [SQab19172] at 0.5 µg/ml dilution.

  • ARG66683 anti-Dengue virus NS4B antibody [SQab19172] WB image

    Western blot: 10 µg of BHK, DENV1-infected BHK, DENV2-infected BHK, DENV3-infected BHK and DENV4-infected BHK cells (left to right). Cell lysates were stained with ARG66683 anti-Dengue virus NS4B antibody [SQab19172] at 0.5 µg/ml dilution.

  • ARG66683 anti-Dengue virus NS4B antibody [SQab19172] WB image

    Western blot: DENV infected (right) or uninfected (left) Huh7 cells. Cell lysates were stained with ARG66683 anti-Dengue virus NS4B antibody [SQab19172] at 1:1000 dilution, overnight.

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