anti-DGCR8 phospho (Ser377) antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
CAT.NO. : ARG52258
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes DGCR8 phospho (Ser377) |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DGCR8 |
| 抗原物种 | Human |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser377 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | DOPA decarboxylase; AADC; DDC; Aromatic-L-amino-acid decarboxylase; EC 4.1.1.28 |
应用说明
| 应用建议 |
| ||||
|---|---|---|---|---|---|
| 应用说明 | Specific for the ~120k DGCR8 protein phosphorylated at Ser377. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # O88533 Mouse Aromatic-L-amino-acid decarboxylase Swiss-port # P20711 Human Aromatic-L-amino-acid decarboxylase |
|---|---|
| 基因名称 | DGCR8 |
| 全名 | dopa decarboxylase (aromatic L-amino acid decarboxylase) |
| 背景介绍 | The Drosha-DGCR8 microprocessor complex is required for microRNA (miRNA) biogenesis. DGCR8 (DiGeorge Syndrome Critical Region 8) recognizes the RNA substrate, whereas Drosha functions as the endonuclease. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, interacts with the pri-miRNA and functions as the molecular anchor that measures the distance from the ds-RNA-ssRNA junction and directs Drosha cleavage 11bp away (Han J et al, 2006). The efficiency of Drosha cleavage increases in the presence of heme and promotes the formation of highly ordered DGCR8 structures upon binding to RNA (Faller et al, 2010). |
| 研究领域 | Cancer antibody; Metabolism antibody; Neuroscience antibody |
| 预测分子量 | 54 kDa |
检测图片 (1)
ARG52258 anti-DGCR8 phospho (Ser377) antibody WB image
Western blot: Mouse nuclei lysate showing specific immunolabeling of the ~120k DGCR8 protein phosphorylated at Ser 377 (control) by using ARG52258 anti-DGCR8 phospho (Ser377) antibody.
The immunolabeling is blocked by the phosphopeptide used as the antigen (Phos pep).
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