anti-E Cadherin antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes E Cadherin
  • 反应物种: Hu, Pig
  • 应用: ELISA, ICC/IF, IHC-Fr, IHC-P
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: E Cadherin
  • 抗原物种: Human
  • 抗原: Recombinant protein encoding aa 600-707 of human E-Cadherin.
  • Brand:
CAT.NO. : ARG52595
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes E Cadherin
反应物种Hu, Pig
应用ELISA, ICC/IF, IHC-Fr, IHC-P
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称E Cadherin
抗原物种Human
抗原Recombinant protein encoding aa 600-707 of human E-Cadherin.
偶联标记Un-conjugated
別名Uvomorulin; Arc-1; Cadherin-1; E-cadherin; CDHE; CD antigen CD324; ECAD; CAM 120/80; LCAM; Epithelial cadherin; UVO; CD324
应用说明
应用建议
应用推荐稀释比
ELISAAssay-Dependent
ICC/IFAssay-Dependent
IHC-FrAssay-Dependent
IHC-P1:30
应用说明IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 30 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照Breast, Skin, Tonsil
属性
形式Liquid
纯化Immunogen affinity purified
缓冲液PBS (pH 7.6), 1% BSA and
抗菌剂
稳定剂1% BSA
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 999 Human CDH1

Swiss-port # P12830 Human Cadherin-1

背景介绍E Cadherin is a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]
生物功能Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.

E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

(Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria. [UniProt]
细胞定位Membrane
产品亮点Related products:
E Cadherin antibodies; E Cadherin Duos / Panels; Anti-Rabbit IgG secondary antibodies;
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研究领域EMT Study antibody; Epithelial Marker antibody
预测分子量97 kDa
翻译后修饰During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.
N-glycosylation at Asn-637 is essential for expression, folding and trafficking.
Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-754.
检测图片 (1)
  • ARG52595 anti-E Cadherin antibody IHC-P image

    Immunohistochemistry: Human Skin stained with ARG52595 anti-E Cadherin antibody.

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