Anti-EGFRvIII Rabbit Monoclonal Antibody [Clone RM419], 31-1305-00 31-1305-02

Key features and details

  • Uniprot: P00533
  • Gene ID: 1956
  • Host Species: Rabbit
  • Species Reactivity: Human
  • Concentration: N/A
  • Brand:
CAT.NO. : 31-1305-00
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Product Details
Product Details
Product NameAnti-EGFR Variant III Rabbit Monoclonal Antibody, Clone RM419
Product DescriptionRabbit monoclonal to Epidermal Growth Factor Receptor Variant III
Catalog No.31-1305-00
31-1305-02
Clone NameRM419
Product Data SheetAnti-EGFR Variant III Rabbit Monoclonal Antibody Data Sheet RM419

Biotinylated Anti-EGFR Variant III Rabbit Monoclonal Antibody Data Sheet RM419
SpecificityRM419 reacts to human Epidermal Growth Factor Receptor Variant III.No cross reactivity with wild type EGF receptor.
ImmunogenA peptide corresponding to epidermal growth factor receptor variant III (EGFRvIII)
ApplicationIHC, WB, ELISA, FC
Species ReactivityHuman
ConjugateNone
Intended UseResearch Use Only
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Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100 µL;
Biotinylated 50 µL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageIHC: 1:100 – 1:200 dilution;
FC: 1:10 – 1:100 dilution;
WB: 1:1000 - 1:2000 dilution;
ELISA: 1:100 – 1:1000 dilution.
Keywords
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Product Images
Immunohistochemical

Immunohistochemical staining of formalin fixed and paraffin embedded human Glioblastoma Multiforme tissue sections with or without EGFRvIIII using anti-EGFRvIII antibody (Clone RM419) at 1:100 dilution.

Western

Western Blot analysis of DKMG (EGFRvIII positive), U118MG, A431, and HeLa whole cell lysates, using anti-EGFRvIII antibody (Clone RM419) and anti-EGFRct (Clone RM294) at 1:1000 dilution.

Flow

Flow cytometry analysis of DKMG and 293 cells, using anti-EGFRvIII antibody (Clone RM419) at 1:50 dilution.

ELISA

ELISA analysis showing RM419 does not react to wild type EGFR protein. The plate was coated with recombinant EGFR variant III and EGFRwt proteins. A serial dilution of anti-EGFRvlll (Clone RM419) or anti-EGFR (Clone RM229) antibodies were used.

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