anti-EGLN2 / PHD1 antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
-
Brand:
CAT.NO. : ARG41862
US＄ Please choose
US＄ Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes EGLN2 / PHD1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | EGLN2 / PHD1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide within aa. 150-250 of Human EGLN2 / PHD1. (NP_444274.1) |
| 偶联标记 | Un-conjugated |
| 別名 | EIT6; HPH-3; HPH-1; HIF-prolyl hydroxylase 1; Egl nine homolog 2; EC 1.14.11.29; Estrogen-induced tag 6; PHD1; Prolyl hydroxylase domain-containing protein 1; HIFPH1; HIF-PH1; Hypoxia-inducible factor prolyl hydroxylase 1 |
应用说明
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HeLa | ||||||
| 实际分子量 | ~ 45 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | EGLN2 |
| 全名 | egl-9 family hypoxia-inducible factor 2 |
| 背景介绍 | The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene. [provided by RefSeq, Feb 2011] |
| 生物功能 | Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. Also regulates susceptibility to normoxic oxidative neuronal death. Links oxygen sensing to cell cycle and primary cilia formation by hydroxylating the critical centrosome component CEP192 which promotes its ubiquitination and subsequent proteasomal degradation. Hydroxylates IKBKB, mediating NF-kappaB activation in hypoxic conditions. Target proteins are preferentially recognized via a LXXLAP motif. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 44 kDa |
检测图片 (2)
ARG41862 anti-EGLN2 / PHD1 antibody WB image
Western blot: 25 µg of HeLa cell lysate stained with ARG41862 anti-EGLN2 / PHD1 antibody at 1:1000 dilution.
ARG41862 anti-EGLN2 / PHD1 antibody IP image
Immunoprecipitation: 150 µg extracts of HeLa cells were immunoprecipitated and stained with ARG41862 anti-EGLN2 / PHD1 antibody at 1:1000 dilution.
New Products
