anti-Eosinophil Major Basic Protein antibody [BMK-13]
![anti-Eosinophil Major Basic Protein antibody [BMK-13]](/upload/image/products/ARG22591_210_205.jpg)
![anti-Eosinophil Major Basic Protein antibody [BMK-13]](/upload/image/products/ARG22591.jpg)
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原物种:
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CAT.NO. : ARG22591
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [BMK-13] recognizes Eosinophil Major Basic Protein This antibody recognizes the Eosinophil Major Basic Protein (EMBP), a 117 amino acid protein, corresponding to residues 106-222 of Bone marrow proteoglycan (precursor). Mouse anti Human Eosinophil Major Basic Protein antibody, clone BMK-13 stains both resting and activated eosinophils of bronchial and skin sections of allergic and normal sites and may be considered a Pan eosinophil marker. Mouse anti Human Eosinophil Major Basic Protein antibody, clone BMK-13 cross reacts weakly with basophils which also contain low levels of EMBP. No cross reactivity with other human cells or proteins has been noted. |
|---|---|
| 反应物种 | Hu, Rat |
| 应用 | IHC-Fr, IHC-P |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | BMK-13 |
| 同位型 | IgG1 |
| 靶点名称 | Eosinophil Major Basic Protein |
| 抗原物种 | Human |
| 偶联标记 | Un-conjugated |
| 別名 | Proteoglycan 2; Bone marrow proteoglycan; MBP1; Pregnancy-associated major basic protein; BMPG; EMBP; MBP |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-Fr: It is recommended that sections are fixed in a 1:1 mixture of acetone and methanol and air-dried for 1 hour. Good results may be achieved via staining with the APAAP method.IHC-P: This product requires enzymatic pre-treatment of paraffin sections prior to staining. Pepsin is recommended for this purpose. NB. Heat-mediated antigen retrieval methods should not be used. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified |
| 缓冲液 | PBS, 0.02% Sodium azide and 0.1% BSA |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.1% BSA |
| 浓度 | 0.1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PRG2 |
| 全名 | proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein) |
| 背景介绍 | The protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin, and in immune hypersensitivity reactions. The encoded protein contains a peptide that displays potent antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria, and fungi. It is directly implicated in epithelial cell damage, exfoliation, and bronchospasm in allergic diseases. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014] |
| 生物功能 | Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA. [UniProt] |
| 预测分子量 | 25 kDa |
| 翻译后修饰 | Nitrated. |
检测图片 (1)
ARG22591 anti-Eosinophil Major Basic Protein antibody [BMK-13] IHC-P image
Immunohistochemistry: Rat duodenumstained with ARG22591 anti-Eosinophil Major Basic Protein antibody [BMK-13].
From Shuai Ji et al. Front Immunol (2022), doi: 10.3389/fimmu.2022.944591, Fig. 5.
参考文献
Multi-omics analysis reveals the metabolic regulators of duodenal low-grade inflammation in a functional dyspepsia model
IHC-P / Rat
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