anti-ERBB2 / HER2 antibody [SQab1890]
![anti-ERBB2 / HER2 antibody [SQab1890]](/upload/image/products/ARG66357_IHC-P_HuBreastCancer2_210_205.jpg)
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Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG66357
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Product Details
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab1890] recognizes ERBB2 / HER2 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab1890 |
| 同位型 | IgG |
| 靶点名称 | ERBB2 / HER2 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the C-terminus of Human HER2 / ErbB2. |
| 偶联标记 | Un-conjugated |
| 別名 | p185erbB2; Proto-oncogene c-ErbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; NGL; EC 2.7.10.1; CD340; CD antigen CD340; TKR1; HER-2; Tyrosine kinase-type cell surface receptor HER2; HER2; NEU; HER-2/neu; MLN 19; Receptor tyrosine-protein kinase erbB-2 |
应用说明
| 应用建议 |
| ||||
|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P04626 Human Receptor tyrosine-protein kinase erbB-2 |
|---|---|
| 基因名称 | ERBB2 |
| 全名 | erb-b2 receptor tyrosine kinase 2 |
| 背景介绍 | This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008] |
| 生物功能 | Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. [UniProt] |
| 产品亮点 | Related products: HER2 antibodies; HER2 ELISA Kits; HER2 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones) |
| 研究领域 | Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody; Circulating Tumor Cells BioMarker antibody |
| 预测分子量 | 138 kDa |
| 翻译后修饰 | Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172). [UniProt] |
检测图片 (2)
ARG66357 anti-ERBB2 / HER2 antibody [SQab1890] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast cancer tissue stained with ARG66357 anti-ERBB2 / HER2 antibody [SQab1890] at 1:500 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).
ARG66357 anti-ERBB2 / HER2 antibody [SQab1890] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast cancer tissue stained with ARG66357 anti-ERBB2 / HER2 antibody [SQab1890] at 1:500 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).
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