anti-Estrogen Receptor beta antibody [57/3]
CAT.NO. : ARG22851
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概述
产品描述 | Mouse Monoclonal antibody [57/3] recognizes Estrogen Receptor beta |
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反应物种 | Hu |
应用 | IHC-P |
宿主 | Mouse |
克隆 | Monoclonal |
克隆号 | 57/3 |
同位型 | IgG1 |
靶点名称 | Estrogen Receptor beta |
抗原物种 | Human |
抗原 | Synthetic peptide around the C-terminus of Human Estrogen Receptor beta2. (CGMKMETLLPEATMEQ) |
偶联标记 | Un-conjugated |
別名 | ER-BETA; NR3A2; ESTRB; Estrogen receptor beta; ER-beta; ESRB; Erb; Nuclear receptor subfamily 3 group A member 2; ESR-BETA |
应用说明
应用建议 |
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应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.2). Glycine/ EDTA preparations have also been used with success in some tissues. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
形式 | Liquid |
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纯化 | Unpurified. |
缓冲液 | PBS and 0.09% Sodium azide |
抗菌剂 | 0.09% Sodium azide |
浓度 | 1 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
数据库连接 | |
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基因名称 | ESR2 |
全名 | estrogen receptor 2 (ER beta) |
背景介绍 | This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008] |
生物功能 | Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. [UniProt] |
预测分子量 | 59 kDa |
翻译后修饰 | Phosphorylation at Ser-87 and Ser-105 recruits NCOA1. |
检测图片 (2)
ARG22851 anti-Estrogen Receptor beta antibody [57/3] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast cancer biopsy stained with ARG22851 anti-Estrogen Receptor beta antibody [57/3]. Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.2). (Low power).
ARG22851 anti-Estrogen Receptor beta antibody [57/3] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast cancer biopsy stained with ARG22851 anti-Estrogen Receptor beta antibody [57/3]. Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.2). (High power).
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