anti-ETFB antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes ETFB
  • 反应物种: Hu, Ms, Rat
  • 预测物种: Cow, Dog
  • 应用: IHC-P, WB
  • 特异性: This antibody is expected to recognize both reported isoforms (NP_001976.1; NP_001014763.1).Amino acid numbering in the name above refers to NP_001976.1).
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: ETFB
  • Brand:
CAT.NO. : ARG65284
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes ETFB
反应物种Hu, Ms, Rat
预测物种Cow, Dog
应用IHC-P, WB
特异性This antibody is expected to recognize both reported isoforms (NP_001976.1; NP_001014763.1).Amino acid numbering in the name above refers to NP_001976.1).
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称ETFB
抗原物种Human
抗原C-PQRTAGVKVETTE
偶联标记Un-conjugated
別名MADD; FP585; Beta-ETF; Electron transfer flavoprotein subunit beta
应用说明
应用建议
应用推荐稀释比
IHC-P3 - 5 µg/ml
WB0.01 - 0.03 µg/ml
应用说明IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 110826 Mouse ETFB

GeneID: 2109 Human ETFB

GeneID: 292845 Rat ETFB

背景介绍This gene encodes electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. The gene deficiencies have been implicated in type II glutaricaciduria. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]
研究领域Cancer antibody; Metabolism antibody; Signaling Transduction antibody
预测分子量28 kDa
翻译后修饰Methylated. Trimethylation at Lys-200 and Lys-203 may negatively regulate the activity in electron transfer from Acyl-CoA dehydrogenases.
检测图片 (2)
  • ARG65284 anti-ETFBantibody WB image

    Western Blot: Mouse Liver lysate (35 µg protein in RIPA buffer) stained with ARG65284 anti-ETFB (aa 231-243) antibody at 0.01 µg/ml dilution.

  • ARG65284 anti-ETFBantibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Liver. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG65284 anti-ETFB (aa 231-243) antibody at 3.8 µg/ml dilution followed by AP-staining.

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