anti-ETFB antibody
CAT.NO. : ARG65284
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概述
产品描述 | Goat Polyclonal antibody recognizes ETFB |
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反应物种 | Hu, Ms, Rat |
预测物种 | Cow, Dog |
应用 | IHC-P, WB |
特异性 | This antibody is expected to recognize both reported isoforms (NP_001976.1; NP_001014763.1).Amino acid numbering in the name above refers to NP_001976.1). |
宿主 | Goat |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | ETFB |
抗原物种 | Human |
抗原 | C-PQRTAGVKVETTE |
偶联标记 | Un-conjugated |
別名 | MADD; FP585; Beta-ETF; Electron transfer flavoprotein subunit beta |
应用说明
应用建议 |
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应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
形式 | Liquid |
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纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
抗菌剂 | 0.02% Sodium azide |
稳定剂 | 0.5% BSA |
浓度 | 0.5 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
数据库连接 | |
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背景介绍 | This gene encodes electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. The gene deficiencies have been implicated in type II glutaricaciduria. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008] |
研究领域 | Cancer antibody; Metabolism antibody; Signaling Transduction antibody |
预测分子量 | 28 kDa |
翻译后修饰 | Methylated. Trimethylation at Lys-200 and Lys-203 may negatively regulate the activity in electron transfer from Acyl-CoA dehydrogenases. |
检测图片 (2)
ARG65284 anti-ETFBantibody WB image
Western Blot: Mouse Liver lysate (35 µg protein in RIPA buffer) stained with ARG65284 anti-ETFB (aa 231-243) antibody at 0.01 µg/ml dilution.
ARG65284 anti-ETFBantibody IHC-P image
Immunohistochemistry: paraffin embedded Human Liver. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG65284 anti-ETFB (aa 231-243) antibody at 3.8 µg/ml dilution followed by AP-staining.
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