anti-F-actin antibody [NH3]

产品描述: Mouse Monoclonal antibody [NH3] recognizes F-actin.
反应物种: Hu, Ms, Rat, Rb
应用: ELISA, FACS, ICC/IF, IHC-Fr, IP, WB
特异性: The clone [NH3] recognises human Filamentous actin (F-actin) and reportedly also recognizes Globular actin (G-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody is recommended to use to detect this primary antibody.
宿主: Mouse
克隆: Monoclonal
克隆号: NH3
同位型: IgM
靶点名称: F-actin

CAT.NO. : ARG23737

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Size:

250 μg

Product Details

概述

产品描述	Mouse Monoclonal antibody [NH3] recognizes F-actin.
反应物种	Hu, Ms, Rat, Rb
应用	ELISA, FACS, ICC/IF, IHC-Fr, IP, WB
特异性	The clone [NH3] recognises human Filamentous actin (F-actin) and reportedly also recognizes Globular actin (G-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody is recommended to use to detect this primary antibody.
宿主	Mouse
克隆	Monoclonal
克隆号	NH3
同位型	IgM
靶点名称	F-actin
抗原物种	Human
抗原	Human monocytes and U937 cell line.
偶联标记	Un-conjugated
別名	PS1TP5BP1; BRWS1; Actin, cytoplasmic 1; Beta-actin

应用说明

应用建议

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purified.
缓冲液	PBS and 0.09% Sodium azide.
抗菌剂	0.09% Sodium azide
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 100009272 Rabbit ACTB GeneID: 11461 Mouse ACTB GeneID: 60 Human ACTB GeneID: 81822 Rat ACTB Swiss-port # P29751 Rabbit Actin, cytoplasmic 1 Swiss-port # P60709 Human Actin, cytoplasmic 1 Swiss-port # P60710 Mouse Actin, cytoplasmic 1 Swiss-port # P60711 Rat Actin, cytoplasmic 1 More
基因名称	ACTB
背景介绍	This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]
生物功能	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. [UniProt]
研究领域	Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody; Cytochrome-C fractionation Study antibody; Inflammation Study antibody; Tag Internal Control antibody
预测分子量	42 kDa

检测图片 (1)

ARG23737 anti-F-actin antibody [NH3] ICC/IF image
Immunofluorescence: Mouse cardiac microvascular endothelial cells were ﬁxed with 4% PFA, permeabilized with 0.3% Triton X-100/PBS and then stained with ARG23737 anti-F-actin antibody [NH3] at 1:100 dilution.

参考文献

Magnetic stirring with iron oxide nanospinners accretes neurotoxic Aβ42 oligomers into phagocytic clearable plaques for Alzheimer's disease treatment

ICC/IF / Mouse

Arjun Sabu et al.
Mater Today Bio., (2024)