anti-FAM129B phospho (Ser679 / Ser683) antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG52278
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes FAM129B phospho (Ser679 / Ser683) |
|---|---|
| 反应物种 | Ms |
| 预测物种 | Hu, NHuPrm |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | FAM129B |
| 抗原物种 | Human |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser679/683 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | MINERVA; bA356B19.6; OC58; Meg-3; Melanoma invasion by ERK; C9orf88; Protein FAM129B; MEG-3; Niban-like protein 1 |
应用说明
| 应用建议 |
| ||||
|---|---|---|---|---|---|
| 应用说明 | Specific for the ~83k FAM129B phosphorylated at Ser679/683 . Immunolabeling is blocked by preadsorption of antibody with the phospho-peptide that was used to geneRate the antibody but not by the corresponding dephospho-peptide. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | FAM129B |
| 全名 | family with sequence similarity 129, member B |
| 背景介绍 | FAM129B, also known as Niban-like protein 1, belongs to a poorly characterized protein family with unknown category and function. Increased expression of the Niban gene has been observed in renal carcinomas (Adachi et al., 2004; Sun et al., 2007). Suppression of FAM129B expression in HeLa cells has been seen to promote apoptosis, suggesting that it can modulate cell death signaling, and may be involved in the ER stress response (Sun et al., 2007). FAM129B is also up-regulated in various types of thyroid tumors and Hashimoto’s thyroiditis (Matsumoto et al., 2006). It has been suggested that the MAP kinase dependent phosphorylation of FAM129B is important in controlling melanoma cells, as inhibition of B/Raf/MKK/ERK in melanoma cells represses invasion (Old et al., 2009). It is believed that phosphorylated FAM129B not only derepresses invasion, but also regulates events that promote invasion (Old et al., 2009). |
| 研究领域 | Cell Biology and Cellular Response antibody |
| 预测分子量 | 84 kDa |
| 翻译后修饰 | Phosphorylated at Ser-641, Ser-646, Ser-692 and Ser-696 by the BRAF/MKK/ERK signaling cascade. In melanoma cells, the C-terminal phosphorylation may prevent targeting to the plasma membrane. As apoptosis proceeds, degraded via an proteasome-independent pathway, probably by caspases. |
检测图片 (1)
ARG52278 anti-FAM129B phospho (Ser679 / Ser683) antibody WB image
Western blot: 3T3 cells showing specific immunolabeling of the ~ 83k FAM129B protein phosphorylated at Ser 679/683 stained with ARG52278 anti-FAM129B phospho (Ser679 / Ser683) antibody.
The phosphospecificity is shown in the second lane where immunoreactivity is blocked by preadsorption with the phospho-peptide (Peptide) used as antigen.
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