anti-FANCD2 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes FANCD2
  • 反应物种: Hu, Rat
  • 应用: ICC/IF, IP, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: FANCD2
  • 抗原物种: Human
  • 抗原: Recombinant fusion protein corresponding to aa. 1-230 of Human FANCD2 (NP_149075.2).
  • Brand:
CAT.NO. : ARG58645
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes FANCD2
反应物种Hu, Rat
应用ICC/IF, IP, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称FANCD2
抗原物种Human
抗原Recombinant fusion protein corresponding to aa. 1-230 of Human FANCD2 (NP_149075.2).
偶联标记Un-conjugated
別名Protein FACD2; FAD2; Fanconi anemia group D2 protein; FACD; FANCD; FA4; FAD; FA-D2
应用说明
应用建议
应用推荐稀释比
ICC/IF1:50 - 1:200
IPAssay-dependent
WB1:500 - 1:2000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照Jurkat
实际分子量175 kDa
属性
形式Liquid
纯化Affinity purified.
缓冲液PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂0.02% Sodium azide
稳定剂50% Glycerol
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 2177 Human FANCD2

GeneID: 312641 Rat FANCD2

Swiss-port # Q6IV68 Rat Fanconi anemia group D2 protein homolog

Swiss-port # Q9BXW9 Human Fanconi anemia group D2 protein

基因名称FANCD2
全名Fanconi anemia, complementation group D2
背景介绍The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group D2. This protein is monoubiquinated in response to DNA damage, resulting in its localization to nuclear foci with other proteins (BRCA1 AND BRCA2) involved in homology-directed DNA repair. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
生物功能Required for maintenance of chromosomal stability. Promotes accurate and efficient pairing of homologs during meiosis. Involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. May participate in S phase and G2 phase checkpoint activation upon DNA damage. Plays a role in preventing breakage and loss of missegregating chromatin at the end of cell division, particularly after replication stress. Required for the targeting, or stabilization, of BLM to non-centromeric abnormal structures induced by replicative stress. Promotes BRCA2/FANCD1 loading onto damaged chromatin. May also be involved in B-cell immunoglobulin isotype switching. [UniProt]
细胞定位Nucleus. [UniProt]
预测分子量164 kDa
翻译后修饰Monoubiquitinated on Lys-561 during S phase and upon genotoxic stress by FANCL in complex with E2 ligases UBE2T or UBE2W (isoform 1 and isoform 2). Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the joint intervention of the FANC core complex, including FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, and FANCM, and proteins involved in cell cycle checkpoints and DNA repair, including RPA1, ATR, CHEK1 and BRCA1, and is mediated by FANCL/PHF9. Ubiquitination is required for binding to chromatin, interaction with BRCA1, BRCA2 and MTMR15/FAN1, DNA repair, and normal cell cycle progression, but not for phosphorylation on Ser-222 or interaction with MEN1.

Phosphorylated in response to various genotoxic stresses by ATM and/or ATR. Upon ionizing radiation, phosphorylated by ATM on Ser-222 and Ser-1404. Phosphorylation on Ser-222 is required for S-phase checkpoint activation, but not for ubiquitination, foci formation, or DNA repair. In contrast, phosphorylation by ATR on other sites may be required for ubiquitination and foci formation. [UniProt]
检测图片 (2)
  • ARG58645 anti-FANCD2 antibody ICC/IF image

    Immunofluorescence: MCF-7 cells stained with ARG58645 anti-FANCD2 antibody.

  • ARG58645 anti-FANCD2 antibody WB image

    Western blot: 25 µg of Jurkat cell lysate stained with ARG58645 anti-FANCD2 antibody at 1:1000 dilution.

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