anti-FEN1 antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
CAT.NO. : ARG54167
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody recognizes FEN1 |
|---|---|
| 反应物种 | Hu, Ms, Rat, Hm, Mk |
| 应用 | ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG1 |
| 靶点名称 | FEN1 |
| 抗原物种 | Human |
| 抗原 | Purified recombinant human FEN-1 protein fragments expressed in E.coli. |
| 偶联标记 | Un-conjugated |
| 別名 | hFEN-1; Maturation factor 1; Flap endonuclease 1; DNase IV; EC 3.1.-.-; FEN-1; MF1; Flap structure-specific endonuclease 1; RAD2 |
应用说明
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | 45 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | FEN1 |
| 全名 | flap structure-specific endonuclease 1 |
| 背景介绍 | Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair.During DNA replication,cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment.It enters the flap from the 5'-end and then tracks to cleave the flap base,leaving a nick for ligation.Also involved in the long patch base excision repair (LP-BER) pathway,by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap.Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions.Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA,and exhibits RNase H activity.Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. |
| 生物功能 | Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. [UniProt] |
| 细胞定位 | Isoform 1: Nucleus > nucleolus. Nucleus > nucleoplasm. Note: Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage. Isoform FENMIT: Mitochondrion. |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 43 kDa |
| 翻译后修饰 | Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300. Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA. Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA. |
检测图片 (2)
ARG54167 anti-FEN1 antibody ICC/IF image
Immunofluorescence: HeLa cells fixed with 4% Paraformaldehyde and stained with ARG54167 anti-FEN1 antibody at 1:400 dilution.
ARG54167 anti-FEN1 antibody WB image
Western blot: HeLa cell lysate stained with ARG54167 anti-FEN1 antibody at 1:1000 dilution.
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