anti-GAP43 antibody [3H14]
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Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
Product Details
| 产品描述 | Mouse Monoclonal antibody [3H14] recognizes GAP43 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-Fr, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 3H14 |
| 同位型 | IgM |
| 靶点名称 | GAP43 |
| 抗原物种 | Human |
| 抗原 | Recombinant full-length Human GAP43. |
| 偶联标记 | Un-conjugated |
| 別名 | pp46; Growth-associated protein 43; B-50; Neuromodulin; PP46; Axonal membrane protein GAP-43; Neural phosphoprotein B-50 |
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification. |
| 缓冲液 | PBS and 50% Glycerol. |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | GAP43 |
| 全名 | growth associated protein 43 |
| 背景介绍 | The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. [UniProt] |
| 预测分子量 | 25 kDa |
| 翻译后修饰 | Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2. |
ARG10715 anti-GAP43 antibody [3H14] ICC/IF image
Immunocytochemistry: Mixed Rat neuronal cultures stained with ARG10715 anti-GAP43 antibody [3H14] (green). The GAP43 antibody stains the plasma membrane of neurons and is particularly concentrated in dendrites.
ARG10715 anti-GAP43 antibody [3H14] ICC/IF image
Immunofluorescence: Cortical neuron-glial cells from E20 Rat stained with ARG10715 anti-GAP43 antibody [3H14] (red) at 1:1000 dilution and costained with ARG52328 anti-MAP2 antibody (green) at 1:10000 dilution. DAPI (blue) for nuclear staining.
The GAP43 antibody labels protein expressed in the axonal membrane of the neuronal cells, while the MAP2 antibody stains dendrites and perikarya of neurons.
ARG10715 anti-GAP43 antibody [3H14] WB image
Western blot: 1) SH-SY5Y cell lysate, and 2) whole Rat brain lysate was stained with ARG10715 anti-GAP43 antibody [3H14] at 1: 2000 dilution.
ARG10715 anti-GAP43 antibody [3H14] WB image
Western blot: Rat brain, Rat spinal cord, Mouse brain, Mouse spinal cord, C6 and SH-SY5Y cell lysates stained with ARG10715 anti-GAP43 antibody [3H14] (green) at 1:5000 dilution.
The protein is expressed in rodent and Human neurons and neuronal derived cells but not in C6 cells which are of glial origin.
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