anti-GCLC antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes GCLC
  • 反应物种: Hu, Ms, Rat
  • 应用: ICC/IF, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: GCLC
  • 抗原物种: Human
  • 抗原: Recombinant fusion protein corresponding to aa. 1-252 of Human GCLC (NP_001489.1).
  • Brand:
CAT.NO. : ARG58109
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes GCLC
反应物种Hu, Ms, Rat
应用ICC/IF, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称GCLC
抗原物种Human
抗原Recombinant fusion protein corresponding to aa. 1-252 of Human GCLC (NP_001489.1).
偶联标记Un-conjugated
別名GCS heavy chain; Gamma-glutamylcysteine synthetase; Gamma-ECS; GCL; GLCL; GCS; Glutamate--cysteine ligase catalytic subunit; EC 6.3.2.2; GLCLC
应用说明
应用建议
应用推荐稀释比
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照Mouse liver
实际分子量~ 75 kDa
属性
形式Liquid
纯化Affinity purified.
缓冲液PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂0.02% Sodium azide
稳定剂50% Glycerol
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 14629 Mouse GCLC

GeneID: 25283 Rat GCLC

GeneID: 2729 Human GCLC

基因名称GCLC
全名glutamate-cysteine ligase, catalytic subunit
背景介绍Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010]
预测分子量73 kDa
检测图片 (1)
  • ARG58109 anti-GCLC antibody WB image

    Western blot: 25 µg of Mouse liver lysate stained with ARG58109 anti-GCLC antibody at 1:3000 dilution.

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