anti-GFAP antibody [5C10]
![anti-GFAP antibody [5C10]](/upload/image/products/ARG11126_WB_1_210_205.jpg)
![anti-GFAP antibody [5C10]](/upload/image/products/ARG11126_IF_1.jpg)
![anti-GFAP antibody [5C10]](/upload/image/products/ARG11126_IHC-Fr_1.jpg)
![anti-GFAP antibody [5C10]](/upload/image/products/ARG11126_WB_1.jpg)
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
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Brand:
Product Details
| 产品描述 | Mouse Monoclonal antibody [5C10] recognizes GFAP |
|---|---|
| 反应物种 | Hu, Ms, Rat, Hrs, Pig |
| 预测物种 | Cow |
| 应用 | ICC/IF, IHC-Fr, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 5C10 |
| 同位型 | IgG1 |
| 靶点名称 | GFAP |
| 抗原物种 | Pig |
| 抗原 | Purified pig spinal cord GFAP. |
| 偶联标记 | Un-conjugated |
| 別名 | Glial fibrillary acidic protein; ALXDRD; GFAP |
| 应用建议 |
| ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | ~ 50 kDa |
| 形式 | Liquid |
|---|---|
| 纯化 | Purified |
| 缓冲液 | PBS, 5 mM Sodium azide and 50% Glycerol. |
| 抗菌剂 | 5 mM Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | GFAP |
| 全名 | glial fibrillary acidic protein |
| 背景介绍 | This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
| 生物功能 | GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt] |
| 细胞定位 | Cytoplasm. Note=Associated with intermediate filaments. [UniProt] |
| 预测分子量 | 50 kDa |
| 翻译后修饰 | Phosphorylated by PKN1. [UniProt] |
ARG11126 anti-GFAP antibody [5C10] ICC/IF image
Immunofluorescence: E20 rat cortical neuron-glial cells stained with ARG11126 anti-GFAP antibody [5C10] (red) at 1:1000 dilution, and co-stained with anti-Neurofilament NF-L antibody (red) at 1:2000 dilution. DAPI (blue) for nuclear staining.
ARG11126 anti-GFAP antibody [5C10] IHC-Fr image
Immunohistochemistry: Frozen section of Rat cerebellum tissue stained with ARG11126 anti-GFAP antibody [5C10] (green) at 1:1000 dilution, and co-stained with ARG10732 anti-Neurofilament NF-L antibody antibody (red) at 1:2000 dilution. (Sample preparation: Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with antibodies.).
ARG11126 anti-GFAP antibody [5C10] WB image
Western blot: Rat brain, Rat spinal cord, Mouse brain and Mouse spinal cord lysates stained with ARG11126 anti-GFAP antibody [5C10] at 1:2000 dilution.
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