anti-GFAP antibody [GA-5]
CAT.NO. : ARG55954
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概述
产品描述 | Mouse Monoclonal antibody [GA-5] recognizes GFAP |
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反应物种 | Hu, Ms, Rat |
应用 | FACS, IHC-P, WB |
宿主 | Mouse |
克隆 | Monoclonal |
克隆号 | GA-5 |
同位型 | IgG1 |
靶点名称 | GFAP |
抗原物种 | Pig |
抗原 | GFAP isolated from Pig spinal cord. |
偶联标记 | Un-conjugated |
別名 | Glial fibrillary acidic protein; ALXDRD; GFAP |
应用说明
应用建议 |
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应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
形式 | Liquid |
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纯化 | Purification with Protein G. |
缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
抗菌剂 | 0.05% Sodium azide |
稳定剂 | 0.1 mg/ml BSA |
浓度 | 0.2 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
数据库连接 | |
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基因名称 | GFAP |
全名 | glial fibrillary acidic protein |
背景介绍 | GFAP is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
生物功能 | GFAP is a class-III intermediate filament. It is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt] |
细胞定位 | Cytoplasmic |
产品亮点 | Related products: GFAP antibodies; GFAP Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Astrocyte-to-neuron conversion for Parkinson's disease treatment |
研究领域 | Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody |
预测分子量 | 50 kDa |
翻译后修饰 | Phosphorylated by PKN1. |
检测图片 (3)
ARG55954 anti-GFAP antibody [GA-5] WB image
Western blot: 20 µg of Mouse brain and Rat brain lysates stained with ARG55954 anti-GFAP antibody [GA-5] at 1:1000 dilution.
ARG55954 anti-GFAP antibody [GA-5] IHC-P image
Immunohistochemistry: Human brain stained with ARG55954 anti-GFAP antibody [GA-5]. Note cytoplasmic staining.
ARG55954 anti-GFAP antibody [GA-5] WB image
Western blot: Human brain lysate stained with ARG55954 anti-GFAP antibody [GA-5].
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