anti-GJC1 / Connexin 45 antibody
产品描述 | Rabbit Polyclonal antibody recognizes GJC1 / Connexin 45 |
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反应物种 | Hu, Ms, Rat |
应用 | IHC-P |
宿主 | Rabbit |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | GJC1 / Connexin 45 |
抗原物种 | Human |
抗原 | Synthetic peptide corresponding to aa. 91-131 of Human GJC1 / Connexin 45. (YLGYAIHKIAKMEHGEADKKAARSKPYAMRWKQHRALEETE) |
偶联标记 | Un-conjugated |
別名 | Cx45; GJA7; Gap junction gamma-1 protein; CX45; Gap junction alpha-7 protein; Connexin-45 |
应用建议 |
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应用说明 | IHC-P: Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
形式 | Liquid |
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纯化 | Affinity purification with immunogen. |
缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
抗菌剂 | 0.05% Sodium azide |
稳定剂 | 5% BSA |
浓度 | 0.5 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
数据库连接 | |
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基因名称 | GJC1 |
全名 | gap junction protein, gamma 1, 45kDa |
背景介绍 | This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Alternatively spliced transcript variants encoding the same isoform have been described. [provided by RefSeq, Jul 2008] |
生物功能 | One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. [UniProt] |
细胞定位 | Cell membrane; Multi-pass membrane protein. Cell junction, gap junction. [UniProt] |
预测分子量 | 45 kDa |
ARG59205 anti-GJC1 / Connexin 45 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse cardiac muscle tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59205 anti-GJC1 / Connexin 45 antibody at 1 µg/ml dilution, overnight at 4°C.
ARG59205 anti-GJC1 / Connexin 45 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat skeletal muscle tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59205 anti-GJC1 / Connexin 45 antibody at 1 µg/ml dilution, overnight at 4°C.
ARG59205 anti-GJC1 / Connexin 45 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59205 anti-GJC1 / Connexin 45 antibody at 1 µg/ml dilution, overnight at 4°C.
ARG59205 anti-GJC1 / Connexin 45 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59205 anti-GJC1 / Connexin 45 antibody at 1 µg/ml dilution, overnight at 4°C.