anti-Glut9 antibody
产品描述 | Rabbit Polyclonal antibody recognizes Glut9 |
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反应物种 | Hu, Rat |
应用 | FACS, IHC-P, WB |
宿主 | Rabbit |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | Glut9 |
抗原物种 | Human |
抗原 | Synthetic peptide corresponding to aa. 262-297 of Human Glut9. (EKHNEARAVKAFQTFLGKADVSQEVEEVLAESRVQR) |
偶联标记 | Un-conjugated |
別名 | Solute carrier family 2, facilitated glucose transporter member 9; GLUT-9; Glucose transporter type 9; UAQTL2; GLUT9; GLUTX; URATv1 |
应用建议 |
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应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min or EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
形式 | Liquid |
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纯化 | Affinity purification with immunogen. |
缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
抗菌剂 | 0.05% Sodium azide |
稳定剂 | 5% BSA |
浓度 | 0.5 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
数据库连接 | Swiss-port # Q9NRM0 Human Solute carrier family 2, facilitated glucose transporter member 9 |
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基因名称 | SLC2A9 |
全名 | solute carrier family 2 (facilitated glucose transporter), member 9 |
背景介绍 | This gene encodes a member of the SLC2A facilitative glucose transporter family. Members of this family play a significant role in maintaining glucose homeostasis. The encoded protein may play a role in the development and survival of chondrocytes in cartilage matrices. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
生物功能 | Urate transporter, which may play a role in the urate reabsorption by proximal tubules (PubMed:18327257, PubMed:28083649). Does not transport glucose, fructose or galactose (PubMed:28083649). [UniProt] |
细胞定位 | Isoform 1: Basolateral cell membrane; Multi-pass membrane protein. Isoform 2: Apical cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. [UniProt] |
预测分子量 | 59 kDa |
ARG43043 anti-Glut9 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human renal cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43043 anti-Glut9 antibody at 1 µg/ml dilution, overnight at 4°C.
ARG43043 anti-Glut9 antibody WB image
Western blot: HepG2 and A549 whole cell lysates stained with ARG43043 anti-Glut9 antibody at 0.5 µg/ml dilution.
ARG43043 anti-Glut9 antibody FACS image
Flow Cytometry: U937 cells were blocked with 10% normal goat serum and then stained with ARG43043 anti-Glut9 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
ARG43043 anti-Glut9 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43043 anti-Glut9 antibody at 2 µg/ml dilution, overnight at 4°C. The section was counterstained with DAPI (blue).
ARG43043 anti-Glut9 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43043 anti-Glut9 antibody at 2 µg/ml dilution, overnight at 4°C. The section was counterstained with DAPI (blue).
ARG43043 anti-Glut9 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestinal tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43043 anti-Glut9 antibody at 2 µg/ml dilution, overnight at 4°C. The section was counterstained with DAPI (blue).