anti-Histone H1 antibody [AE-4]
产品描述 | Mouse Monoclonal antibody [AE-4] recognizes Histone H1 |
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反应物种 | Hu, Ms, Rat |
应用 | FACS, ICC/IF, IHC-P |
特异性 | This clone is a good antibody for nucleus marker staining. This clone reacts Histone H1 at 31 kDa, and might cross-react to other Histone protein around 17 Kda. |
宿主 | Mouse |
克隆 | Monoclonal |
克隆号 | AE-4 |
同位型 | IgG2a, kappa |
靶点名称 | Histone H1 |
抗原物种 | Human |
抗原 | Nuclei of Human leukemia biopsy cells. |
偶联标记 | Un-conjugated |
別名 | Histone H1.0; H1FV; H10; 0; Histone H1; Histone H1' |
应用建议 |
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应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
形式 | Liquid |
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纯化 | Purification with Protein G. |
缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
抗菌剂 | 0.05% Sodium azide |
稳定剂 | 0.1 mg/ml BSA |
浓度 | 0.2 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
数据库连接 | |
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基因名称 | H1F0 |
全名 | H1 histone family, member 0 |
背景介绍 | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H1 family. [provided by RefSeq, Jul 2008] |
生物功能 | Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. [UniProt] |
细胞定位 | Nuclear |
预测分子量 | 21 kDa |
翻译后修饰 | Phosphorylated on Ser-17 in RNA edited version. ADP-ribosylated on Ser-104 in response to DNA damage. |
ARG56026 anti-Histone H1 antibody [AE-4] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human ovarian carcinoma stained with ARG56026 anti-Histone H1 antibody [AE-4].
ARG56026 anti-Histone H1 antibody [AE-4] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human tonsil stained with ARG56026 anti-Histone H1 antibody [AE-4].
ARG56026 anti-Histone H1 antibody [AE-4] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human angiosarcoma stained with ARG56026 anti-Histone H1 antibody [AE-4].
ARG56026 anti-Histone H1 antibody [AE-4] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human testicular carcinoma stained with ARG56026 anti-Histone H1 antibody [AE-4].
ARG56026 anti-Histone H1 antibody [AE-4] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human pancreas stained with ARG56026 anti-Histone H1 antibody [AE-4].