anti-Histone H1 antibody [AE-4]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [AE-4] recognizes Histone H1
  • 反应物种: Hu, Ms, Rat
  • 应用: FACS, ICC/IF, IHC-P
  • 特异性: This clone is a good antibody for nucleus marker staining. This clone reacts Histone H1 at 31 kDa, and might cross-react to other Histone protein around 17 Kda.
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: AE-4
  • 同位型: IgG2a, kappa
  • 靶点名称: Histone H1
  • Brand:
CAT.NO. : ARG56026
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Product Details
概述
产品描述Mouse Monoclonal antibody [AE-4] recognizes Histone H1
反应物种Hu, Ms, Rat
应用FACS, ICC/IF, IHC-P
特异性This clone is a good antibody for nucleus marker staining. This clone reacts Histone H1 at 31 kDa, and might cross-react to other Histone protein around 17 Kda.
宿主Mouse
克隆Monoclonal
克隆号AE-4
同位型IgG2a, kappa
靶点名称Histone H1
抗原物种Human
抗原Nuclei of Human leukemia biopsy cells.
偶联标记Un-conjugated
別名Histone H1.0; H1FV; H10; 0; Histone H1; Histone H1'
应用说明
应用建议
应用推荐稀释比
FACS1 - 2 µg/10^6 cells
ICC/IF1 - 2 µg/ml
IHC-P1 - 2 µg/ml
应用说明IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purification with Protein G.
缓冲液PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
抗菌剂0.05% Sodium azide
稳定剂0.1 mg/ml BSA
浓度0.2 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 14958 Mouse H1F0

GeneID: 24437 Rat H1F0

GeneID: 3005 Human H1F0

基因名称H1F0
全名H1 histone family, member 0
背景介绍Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H1 family. [provided by RefSeq, Jul 2008]
生物功能Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. [UniProt]
细胞定位Nuclear
预测分子量21 kDa
翻译后修饰Phosphorylated on Ser-17 in RNA edited version.
ADP-ribosylated on Ser-104 in response to DNA damage.
检测图片 (5)
  • ARG56026 anti-Histone H1 antibody [AE-4] IHC-P image

    Immunohistochemistry: Formalin-fixed, paraffin-embedded Human ovarian carcinoma stained with ARG56026 anti-Histone H1 antibody [AE-4].

  • ARG56026 anti-Histone H1 antibody [AE-4] IHC-P image

    Immunohistochemistry: Formalin-fixed, paraffin-embedded Human tonsil stained with ARG56026 anti-Histone H1 antibody [AE-4].

  • ARG56026 anti-Histone H1 antibody [AE-4] IHC-P image

    Immunohistochemistry: Formalin-fixed, paraffin-embedded Human angiosarcoma stained with ARG56026 anti-Histone H1 antibody [AE-4].

  • ARG56026 anti-Histone H1 antibody [AE-4] IHC-P image

    Immunohistochemistry: Formalin-fixed, paraffin-embedded Human testicular carcinoma stained with ARG56026 anti-Histone H1 antibody [AE-4].

  • ARG56026 anti-Histone H1 antibody [AE-4] IHC-P image

    Immunohistochemistry: Formalin-fixed, paraffin-embedded Human pancreas stained with ARG56026 anti-Histone H1 antibody [AE-4].

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