Anti-Histone H3.3 G34V Rabbit Monoclonal Antibody [Clone RM307]; Histone H3.3 G34V Mutant; 31-1193-00-S; 31-1193-00-L; 31-1193-02
![Anti-Histone H3.3 G34V Rabbit Monoclonal Antibody [Clone RM307]; Histone H3.3 G34V Mutant; 31-1193-00-S; 31-1193-00-L; 31-1193-02](/upload/image/products/Fig3_RM193_IHC_G34V.png)
![Anti-Histone H3.3 G34V Rabbit Monoclonal Antibody [Clone RM307]; Histone H3.3 G34V Mutant; 31-1193-00-S; 31-1193-00-L; 31-1193-02](/upload/image/products/Fig2_IHC_G34V.png)
![Anti-Histone H3.3 G34V Rabbit Monoclonal Antibody [Clone RM307]; Histone H3.3 G34V Mutant; 31-1193-00-S; 31-1193-00-L; 31-1193-02](/upload/image/products/Fig1_WB_G34V.png)
Key features and details
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CAT.NO. : 31-1193-00
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Product Details
Product Details
| Product Name | Anti-Histone H3.3 G34V Rabbit Monoclonal Antibody, Clone RM307 |
| Product Description | Rabbit monoclonal to Histone H3.3 G34V; Histone H3.3 G34V Mutant |
| Catalog No. | 31-1193-00-S; 31-1193-00-L; 31-1193-02 |
| Clone Name | RM307 |
| Product Data Sheet | Anti-Histone H3.3 G34V Mutant Rabbit Monoclonal Antibody Data Sheet RM307 Biotinylated Anti-Histone H3.3 G34V Mutant Rabbit Monoclonal Antibody Data Sheet RM307 |
| Specificity | RM307 reacts to the Histone H3.3 G34V mutant. No cross reactivity with wild type Histone H3.3 . |
| Immunogen | A peptide corresponding to Histone H3.3 G34V mutant |
| Application | Western Blot ELISA Immunohistochemistry |
| Species Reactivity | All |
| Conjugate | None |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µL; 400 µL; Biotinylated 50 µL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 1:1000 – 1:10000; ELISA: 1:500 – 1:5000; IHC: 1:100 - 1:500. |
| References for Histone H3.3 G34V Mutant antibody [RM307] | Nambiraja A et al C19MC amplification and expression of Lin28A and Olig2 in the classification of embryonal tumors of the central nervous system: A 14-year retrospective study from a tertiary care center. Childs Nerv Syst. (2021), 10.1007/s00381-020-04973-0. IHC; Human. Read more (PubMed: 33236184) Minasi S et al Alternative lengthening of telomeres in molecular subgroups of paediatric high-grade glioma. Childs Nerc Syst. (2020), 10.1007/s00381-020-04933-8. IHC; Human. Read more (PubMed: 33128602) Sangatsuda Y et al. Base-resolution methylomes of gliomas bearing histone H3.3 mutations reveal a G34 mutant-specific signature shared with bone tumors. Neuro Oncol. (2020), 10.1093/neuonc/noaa222.836. WB; Human. Read more (PubMed: PMC7715604) Ito Y et al. Giant cell-rich osteosarcoma of the vertebra with murine double minute chromosome 2- and cyclin-dependent kinase 4-positive and histone H3F3A mutant p.Gly34Trp-negative immunophenotypes. Pathol Int. (2018), 10.1111/pin.12650. IHC; Human. Read more (PubMed: 29446514) Yamamoto H et al. Diagnostic utility of histone H3.3G34 W, G34R, and G34 V mutant-specific antibodies for giant cell tumors of bone. Human Pathology (2017) 10.1016/j.humpath.2017.11.020. IHC; Human. Read more (PubMed: 29241742) |
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Product Images
| Immunohistochemical staining of Giant Cell Bone Tumor (GCBT) tissue sections using Anti-Histone H3.3 G34V antibody, Clone RM307. Image courtesy of Hidetaka Yamamoto, MD, PhD, Kyushu University, Fukuoka, Japan |
 | Immunohistochemical staining of formalin fixed and paraffin embedded 293T cells transfected with a DNA construct encoding Histone H3.3 wild type or G34V mutant, stained with anti-Histone H3.3 G34V rabbit monoclonal antibody, Clone RM307. |
 | Western Blot analysis of cell lysates prepared from 293T, transfected with a DNA construct encoding wild type or G34V mutant proteins of Histone H3.3, using anti-Histone H3.3 G34V rabbit monoclonal antibody, Clone RM307. |
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