Anti-Histone H3.3 G34V Rabbit Monoclonal Antibody [Clone RM307]; Histone H3.3 G34V Mutant; 31-1193-00-S; 31-1193-00-L; 31-1193-02

Key features and details

Uniprot: P84243
Gene ID: 8350
Host Species: Rabbit
Species Reactivity: Human
Concentration: N/A

Brand:

CAT.NO. : 31-1193-02

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Size:

50 µL 100 µL 400 µL

Conjugation:

Unconjugated Biotin Conjugate

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Product Details


Product Name	Anti-Histone H3.3 G34V Rabbit Monoclonal Antibody, Clone RM307
Product Description	Rabbit monoclonal to Histone H3.3 G34V; Histone H3.3 G34V Mutant
Catalog No.	31-1193-00-S; 31-1193-00-L; 31-1193-02
Clone Name	RM307
Product Data Sheet	Anti-Histone H3.3 G34V Mutant Rabbit Monoclonal Antibody Data Sheet RM307 Biotinylated Anti-Histone H3.3 G34V Mutant Rabbit Monoclonal Antibody Data Sheet RM307
Specificity	RM307 reacts to the Histone H3.3 G34V mutant. No cross reactivity with wild type Histone H3.3 .
Immunogen	A peptide corresponding to Histone H3.3 G34V mutant
Application	Western Blot ELISA Immunohistochemistry
Species Reactivity	All
Conjugate	None
Intended Use	Research Use Only
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Properties

Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	100 µL; 400 µL; Biotinylated 50 µL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Western Blot: 1:1000 – 1:10000; ELISA: 1:500 – 1:5000; IHC: 1:100 - 1:500.
References for Histone H3.3 G34V Mutant antibody [RM307]	Nambiraja A et al C19MC amplification and expression of Lin28A and Olig2 in the classification of embryonal tumors of the central nervous system: A 14-year retrospective study from a tertiary care center. Childs Nerv Syst. (2021), 10.1007/s00381-020-04973-0. IHC; Human. Read more (PubMed: 33236184) Minasi S et al Alternative lengthening of telomeres in molecular subgroups of paediatric high-grade glioma. Childs Nerc Syst. (2020), 10.1007/s00381-020-04933-8. IHC; Human. Read more (PubMed: 33128602) Sangatsuda Y et al. Base-resolution methylomes of gliomas bearing histone H3.3 mutations reveal a G34 mutant-specific signature shared with bone tumors. Neuro Oncol. (2020), 10.1093/neuonc/noaa222.836. WB; Human. Read more (PubMed: PMC7715604) Ito Y et al. Giant cell-rich osteosarcoma of the vertebra with murine double minute chromosome 2- and cyclin-dependent kinase 4-positive and histone H3F3A mutant p.Gly34Trp-negative immunophenotypes. Pathol Int. (2018), 10.1111/pin.12650. IHC; Human. Read more (PubMed: 29446514) Yamamoto H et al. Diagnostic utility of histone H3.3G34 W, G34R, and G34 V mutant-specific antibodies for giant cell tumors of bone. Human Pathology (2017) 10.1016/j.humpath.2017.11.020. IHC; Human. Read more (PubMed: 29241742)
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Product Images

Immunohistochemical staining of Giant Cell Bone Tumor (GCBT) tissue sections using Anti-Histone H3.3 G34V antibody, Clone RM307.

Image courtesy of Hidetaka Yamamoto, MD, PhD, Kyushu University, Fukuoka, Japan

Immunohistochemical staining of formalin fixed and paraffin embedded 293T cells transfected with a DNA construct encoding Histone H3.3 wild type or G34V mutant, stained with anti-Histone H3.3 G34V rabbit monoclonal antibody, Clone RM307.

Western Blot analysis of cell lysates prepared from 293T, transfected with a DNA construct encoding wild type or G34V mutant proteins of Histone H3.3, using anti-Histone H3.3 G34V rabbit monoclonal antibody, Clone RM307.

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