anti-HLA Class I antibody [MEM-147] (PE)

Key features and details

  • 产品描述: PE-conjugated Mouse Monoclonal antibody [MEM-147] recognizes HLA Class I
  • 反应物种: Hu
  • 应用: FACS
  • 特异性: The clone MEM-147 reacts with all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: MEM-147
  • 同位型: IgG1
  • 靶点名称: HLA Class I
  • Brand:
CAT.NO. : ARG53936
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Product Details
概述
产品描述PE-conjugated Mouse Monoclonal antibody [MEM-147] recognizes HLA Class I
反应物种Hu
应用FACS
特异性The clone MEM-147 reacts with all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types.
MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.
宿主Mouse
克隆Monoclonal
克隆号MEM-147
同位型IgG1
靶点名称HLA Class I
抗原PHA-activated peripheral blood lymphocytes
偶联标记PE
別名MHC class I antigen A*1; HLAA; HLA class I histocompatibility antigen, A-1 alpha chain
应用说明
应用建议
应用推荐稀释比
FACS1 - 2 µg/ml
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化说明The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
缓冲液PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
抗菌剂15 mM Sodium azide
稳定剂0.2% (w/v) high-grade protease free BSA
浓度0.1 mg/ml
存放说明Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 3105 Human HLA-A

Swiss-port # P30443 Human HLA class I histocompatibility antigen, A-1 alpha chain

基因名称HLA-A
全名major histocompatibility complex, class I, A
背景介绍HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteinsexpressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
生物功能Involved in the presentation of foreign antigens to the immune system. [UniProt]
研究领域Immune System antibody
预测分子量40 kDa
翻译后修饰Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system (By similarity).
检测图片 (1)
  • ARG53936 anti-HLA Class I antibody [MEM-147] (PE) FACS image

    Flow Cytometry: Human peripheral blood stained with ARG53936 anti-HLA Class I antibody [MEM-147] (PE).

参考文献

克隆号文献

The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex.

Tran TM et al.
Immunogenetics.,  (2001)

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