anti-HLA E antibody [MEM-E/06]

产品描述: Mouse Monoclonal antibody [MEM-E/06] recognizes HLA E
反应物种: Hu, NHuPrm
应用: FACS, IHC-P, IP
特异性: The clone MEM-E/06 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results showed that the antibody cross-reacts with some classical MHC Class I molecules (HLA-A3, -A11, -B7). However, the recent Workshop I Session on the 3rd International Conference on HLA-G (Paris, July 2003) confirmed that the antibody exhibits much broader cross-reactivity classical MHC Class I antigens, namely with HLA-A24, -A32, -B8, -B15, -B27, -B35, -B44, -B54, -C3, -C4, -C5, -C7.
宿主: Mouse
克隆: Monoclonal
克隆号: MEM-E/06
同位型: IgG1
靶点名称: HLA E

CAT.NO. : ARG63018

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Size:

100 μg

Product Details

概述

产品描述	Mouse Monoclonal antibody [MEM-E/06] recognizes HLA E
反应物种	Hu, NHuPrm
应用	FACS, IHC-P, IP
特异性	The clone MEM-E/06 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results showed that the antibody cross-reacts with some classical MHC Class I molecules (HLA-A3, -A11, -B7). However, the recent Workshop I Session on the 3rd International Conference on HLA-G (Paris, July 2003) confirmed that the antibody exhibits much broader cross-reactivity classical MHC Class I antigens, namely with HLA-A24, -A32, -B8, -B15, -B27, -B35, -B44, -B54, -C3, -C4, -C5, -C7.
宿主	Mouse
克隆	Monoclonal
克隆号	MEM-E/06
同位型	IgG1
靶点名称	HLA E
抗原	Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.
偶联标记	Un-conjugated
別名	MHC class I antigen E; QA1; EA2.1; HLA-6.2; EA1.2; MHC; HLA class I histocompatibility antigen, alpha chain E

应用说明

应用建议

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

阳性对照

IHC-P: Spleen.

属性

形式	Liquid
纯化	Purified from ascites by protein-A affinity chromatography.
纯度	> 95% (by SDS-PAGE)
缓冲液	PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂	15 mM Sodium azide
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 3133 Human HLA-E Swiss-port # P13747 Human HLA class I histocompatibility antigen, alpha chain E
基因名称	HLA-E
全名	major histocompatibility complex, class I, E
背景介绍	HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. [provided by RefSeq, Jul 2008]
生物功能	Preferably binds to a peptide derived from the signal sequence of most HLA-A, -B, -C and -G molecules. [UniProt]
研究领域	Immune System antibody
预测分子量	40 kDa

检测图片 (1)

ARG63018 anti-HLA E antibody [MEM-E/06] IHC-P image
Immunohistochemistry: Human spleen (paraffin sections) stained with ARG63018 anti-HLA E antibody [MEM-E/06].

参考文献

克隆号文献

HLA-E monoclonal antibodies recognize shared peptide sequences on classical HLA class Ia: relevance to human natural HLA antibodies.

ELISA / Human

Ravindranath MH et al.
Mol Immunol., (2010)