anti-HRP antibody [SQab1887]

Key features and details

  • 产品描述: Recombinant Rabbit Monoclonal antibody [SQab1887] recognizes HRP
  • 反应物种: Other
  • 应用: FACS, ICC/IF, WB
  • 宿主: Rabbit
  • 克隆: Monoclonal
  • 克隆号: SQab1887
  • 同位型: IgG
  • 靶点名称: HRP
  • 抗原物种: Others
  • Brand:
CAT.NO. : ARG66339
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Product Details
概述
产品描述Recombinant Rabbit Monoclonal antibody [SQab1887] recognizes HRP
反应物种Other
应用FACS, ICC/IF, WB
宿主Rabbit
克隆Monoclonal
克隆号SQab1887
同位型IgG
靶点名称HRP
抗原物种Others
抗原Full length native protein (purified) corresponding to HRP.
偶联标记Un-conjugated
应用说明
应用建议
应用推荐稀释比
FACS1:50
ICC/IF1:200
WB1:5000 - 1:10000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purification with Protein A.
缓冲液PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂0.01% Sodium azide
稳定剂40% Glycerol and 0.05% BSA
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
预测分子量39 kDa
检测图片 (3)
  • ARG66339 anti-HRP antibody [SQab1887] ICC/IF image

    Immunofluorescence: HeLa cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66339 anti-HRP antibody [SQab1887] (green) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

  • ARG66339 anti-HRP antibody [SQab1887] FACS image

    Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66339 anti-HRP antibody [SQab1887] (red) at 1:50 dilution in 1x PBS/1% BSA for 60 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.

  • ARG66339 anti-HRP antibody [SQab1887] WB image

    Western blot: 20 ng of HRP protein stained with ARG66339 anti-HRP antibody [SQab1887] at 1:10000 dilution.

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