anti-Hsp 27 antibody [5D12-A12]

产品描述: Mouse Monoclonal antibody [5D12-A12] recognizes Hsp 27
反应物种: Hu
应用: ELISA, ICC/IF, IHC, IP, WB
特异性: Detects ~27kDa. Has no cross-reactivity to Alpha B crystallin. Very limited cross-reactivity to other species.
宿主: Mouse
克隆: Monoclonal
克隆号: 5D12-A12
同位型: IgG2b, kappa
靶点名称: Hsp 27

CAT.NO. : ARG22218

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Size:

100 μg

Product Details

概述

产品描述	Mouse Monoclonal antibody [5D12-A12] recognizes Hsp 27
反应物种	Hu
应用	ELISA, ICC/IF, IHC, IP, WB
特异性	Detects ~27kDa. Has no cross-reactivity to Alpha B crystallin. Very limited cross-reactivity to other species.
宿主	Mouse
克隆	Monoclonal
克隆号	5D12-A12
同位型	IgG2b, kappa
靶点名称	Hsp 27
抗原物种	Human
抗原	Human HSP27
偶联标记	Un-conjugated
別名	HSP 27; Heat shock 27 kDa protein; HMN2B; HS.76067; SRP27; HEL-S-102; HspB1; CMT2F; 28 kDa heat shock protein; HSP27; Heat shock protein beta-1; Hsp25; Estrogen-regulated 24 kDa protein; Stress-responsive protein 27; HSP28

应用说明

应用建议

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein G.
缓冲液	PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
抗菌剂	0.09% Sodium azide
稳定剂	50% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 3315 Human HSPB1 Swiss-port # P04792 Human Heat shock protein beta-1
基因名称	HSPB1
全名	heat shock 27kDa protein 1
背景介绍	The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]
生物功能	Involved in stress resistance and actin organization. [UniProt]
细胞定位	Cytoplasm, cytoskeleton, Nucleus, spindle
预测分子量	23 kDa
翻译后修饰	Phosphorylated upon exposure to protein kinase C activators and heat shock (PubMed:8325890). Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement (PubMed:1332886, PubMed:8093612, PubMed:19166925).

检测图片 (3)

ARG22218 anti-Hsp 27 antibody [5D12-A12] ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22218 anti-Hsp 27 antibody [5D12-A12] (green) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
ARG22218 anti-Hsp 27 antibody [5D12-A12] WB image
Western blot: Human cell lysates stained with ARG22218 anti-Hsp 27 antibody [5D12-A12] at 1:1000 dilution.
ARG22218 anti-Hsp 27 antibody [5D12-A12] ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22218 anti-Hsp 27 antibody [5D12-A12] (green) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.

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