anti-Hsp 60 antibody

Key features and details

  • 产品描述: Mouse Monoclonal antibody recognizes Hsp 60
  • 反应物种: Hu, Ms, Rat
  • 应用: ICC/IF, IP, WB
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 同位型: IgG2b
  • 靶点名称: Hsp 60
  • 抗原物种: Human
  • 抗原: Purified recombinant human Hsp60 protein fragments expressed in E.coli.
  • Brand:
CAT.NO. : ARG54173
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Product Details
概述
产品描述Mouse Monoclonal antibody recognizes Hsp 60
反应物种Hu, Ms, Rat
应用ICC/IF, IP, WB
宿主Mouse
克隆Monoclonal
同位型IgG2b
靶点名称Hsp 60
抗原物种Human
抗原Purified recombinant human Hsp60 protein fragments expressed in E.coli.
偶联标记Un-conjugated
別名Heat shock protein 60; HuCHA60; 60 kDa heat shock protein, mitochondrial; GROEL; P60 lymphocyte protein; 60 kDa chaperonin; HSP-60; Mitochondrial matrix protein P1; HLD4; SPG13; HSP60; Chaperonin 60; Hsp60; CPN60; HSP65
应用说明
应用建议
应用推荐稀释比
ICC/IF1:100
IP1:100
WB1:1000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purified
缓冲液PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol
抗菌剂0.02% Sodium azide
稳定剂50% Glycerol
浓度2.6 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 15510 Mouse HSPD1

GeneID: 3329 Human HSPD1

GeneID: 63868 Rat HSPD1

基因名称HSPD1
全名heat shock 60kDa protein 1 (chaperonin)
背景介绍Implicated in mitochondrial protein import and macromolecular assembly.May facilitate the correct folding of imported proteins.May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
生物功能Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. [UniProt]
细胞定位Mitochondrion matrix.
产品亮点Related Antibody Duos and Panels:
ARG30271 Mitochondrial Marker Antibody Panel (Cytochrome C, COX4, HSP60)
Related products:
Hsp60 antibodies; Hsp60 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related poster download:
The Structure & Functions of Mitochondria.pdf
研究领域Controls and Markers antibody; Signaling Transduction antibody; Mitochondrial Marker antibody
预测分子量61 kDa
检测图片 (4)
  • ARG54173 anti-Hsp 60 antibody ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG54173 anti-Hsp 60 antibody at 1:100 dilution. Left: primary antibody (green). Middle: DAPI (blue). Right: Merge.

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

  • ARG54173 anti-Hsp 60 antibody WB image

    Western blot: 20 µg of 293T, Mouse brain and Rat brain lysates stained with ARG54173 anti-Hsp 60 antibody at 1:1000 dilution.

  • ARG54173 anti-Hsp 60 antibody WB image

    Western blot: 15 µg of H1299, U87, HeLa, Jurkat and Raw264.7 cell lysates stained with ARG54173 anti-Hsp 60 antibody.

  • ARG54173 anti-Hsp 60 antibody IP image

    Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG54173 anti-Hsp 60 antibody.

参考文献

RSV replication is promoted by autophagy-mediated inhibition of apoptosis.

WB / Human

Li Miao et al.
J Virol.,  (2018)

publication_link

 

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