anti-IL10 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes IL10
  • 反应物种: Ms
  • 应用: ELISA, Neut, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: IL10
  • 抗原物种: Mouse
  • 抗原: E.coli derived Recombinant Mouse IL-10.(MSRGQYSRED NNCTHFPVGQ SHMLLELRTA FSQVKTFFQT KDQLDNILLT DSLMQDFKGY LGCQALSEMI QFYLVEVMPQ AEKHGPEIKE HLNSLGQKLK TLRMRLRRCH RFLPCENKSK AVEQVKSDFN KLEDQGVYKA MNEFDIFINC IEAYMMIKMK S)
  • Brand:
CAT.NO. : ARG56654
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes IL10
反应物种Ms
应用ELISA, Neut, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称IL10
抗原物种Mouse
抗原E.coli derived Recombinant Mouse IL-10.
(MSRGQYSRED NNCTHFPVGQ SHMLLELRTA FSQVKTFFQT KDQLDNILLT DSLMQDFKGY LGCQALSEMI QFYLVEVMPQ AEKHGPEIKE HLNSLGQKLK TLRMRLRRCH RFLPCENKSK AVEQVKSDFN KLEDQGVYKA MNEFDIFINC IEAYMMIKMK S)
偶联标记Un-conjugated
別名IL10A; GVHDS; TGIF; IL-10; Cytokine synthesis inhibitory factor; CSIF; Interleukin-10
应用说明
应用建议
应用推荐稀释比
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56764 as a detection antibody
Neut0.043 - 0.064 µg/ml (To yield [ND50] of the biological activity of mIL - 10 (1.0 ng/ml) )
WB0.1 - 0.2 µg/ml
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液PBS (pH 7.2)
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 16153 Mouse IL10

Swiss-port # P18893 Mouse Interleukin-10

基因名称Il10
全名interleukin 10
背景介绍The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]
生物功能Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. [UniProt]
预测分子量21 kDa
检测图片 (3)
  • ARG56654 anti-IL10 antibody WB image

    Western blot: 250 - 0.24 ng of Mouse IL-10 stained with ARG56654 anti-IL10 antibody, under reducing conditions.

  • ARG56654 anti-IL10 antibody WB image

    Western blot: 250 - 0.24 ng of Mouse IL-10 stained with ARG56654 anti-IL10 antibody, under non-reducing conditions.

  • ARG56654 anti-IL10 antibody standard curve image

    Sandwich ELISA: ARG56654 anti-IL10 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56764 anti-IL10 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.

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