anti-IL4 antibody [1.3_4F11-2G3]

CAT.NO. : ARG56588

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Size:

200 μg 100 μg

Product Details

概述

产品描述	Mouse Monoclonal antibody [1.3_4F11-2G3] recognizes IL4
反应物种	Hu, Ms
应用	ELISA, WB
宿主	Mouse
克隆	Monoclonal
克隆号	1.3_4F11-2G3
同位型	IgG1, kappa
靶点名称	IL4
抗原物种	Human
抗原	E.coli derived Recombinant Human IL-4. (MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS)
偶联标记	Un-conjugated
別名	BSF-1; IL-4; B-cell stimulatory factor 1; BCGF1; Lymphocyte stimulatory factor 1; BCGF-1; Interleukin-4; Pitrakinra; Binetrakin; BSF1

应用说明

应用建议

应用	推荐稀释比
ELISA	Sandwich: 0.5 - 1.0 µg/ml with ARG56724 as a detection antibody
WB	0.20 - 0.50 µg/ml

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein A.
缓冲液	PBS (pH 7.2)
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 16189 Mouse IL4 GeneID: 3565 Human IL4 Swiss-port # P05112 Human Interleukin-4 Swiss-port # P07750 Mouse Interleukin-4
基因名称	IL4
全名	interleukin 4
背景介绍	The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
生物功能	Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. [UniProt]
预测分子量	17 kDa

检测图片 (3)

ARG56588 anti-IL4 antibody [1.3_4F11-2G3] WB image
Western blot: 250 - 0.24 ng (left to right) of Human IL-4 stained with ARG56588 anti-IL4 antibody [1.3_4F11-2G3], under reducing conditions.
ARG56588 anti-IL4 antibody [1.3_4F11-2G3] WB image
Western blot: 250 - 0.24 ng (left to right) of Human IL-4 stained with ARG56588 anti-IL4 antibody [1.3_4F11-2G3], under non-reducing conditions.
ARG56588 anti-IL4 antibody [1.3_4F11-2G3] standard curve image
Sandwich ELISA: ARG56588 anti-IL4 antibody [1.3_4F11-2G3] as a capture antibody at 0.5 - 1.0 µg/ml combined with ARG56724 anti-IL4 antibody (Biotin) as a detection antibody at ~ 2.0 - 4.0 µg/ml. Results of a typical standard run with optical density.

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