anti-Liver FABP antibody [2G4]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [2G4] recognizes Liver FABP
  • 反应物种: Hu
  • 应用: FACS, ICC/IF, IHC-P, WB
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: 2G4
  • 同位型: IgG1, kappa
  • 靶点名称: Liver FABP
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG56964
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Product Details
概述
产品描述Mouse Monoclonal antibody [2G4] recognizes Liver FABP
反应物种Hu
应用FACS, ICC/IF, IHC-P, WB
宿主Mouse
克隆Monoclonal
克隆号2G4
同位型IgG1, kappa
靶点名称Liver FABP
抗原物种Human
抗原Recombinant fragment around aa. 1-127 of Human Liver FABP.
偶联标记Un-conjugated
別名FABPL; Fatty acid-binding protein, liver; Liver-type fatty acid-binding protein; L-FABP; Fatty acid-binding protein 1
应用说明
应用建议
应用推荐稀释比
FACSAssay-dependent
ICC/IF1:100
IHC-P1:100
WB1:1000 - 1:2000
应用说明IHC-P: Antigen Retrieval: Boil tissue section in 0.1M Sodium citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purification with Protein G.
缓冲液PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂0.02% Sodium azide
稳定剂10% Glycerol
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 2168 Human FABP1

Swiss-port # P07148 Human Fatty acid-binding protein, liver

基因名称FABP1
全名fatty acid binding protein 1, liver
背景介绍This gene encodes the fatty acid binding protein found in liver. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. This protein and FABP6 (the ileal fatty acid binding protein) are also able to bind bile acids. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq, Mar 2011]
生物功能Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport. [UniProt]
预测分子量14 kDa
检测图片 (4)
  • ARG56964 anti-Liver FABP antibody [2G4] ICC/IF image

    Immunoflorescense: Hep3B cell line stained with ARG56964 anti-Liver FABP antibody [2G4] at 1:100 (Green).

    DAPI (Blue) for nucleus staining.

  • ARG56964 anti-Liver FABP antibody [2G4] FACS image

    Flow Cytometry: Hep3B cell line stained with ARG56964 anti-Liver FABP antibody [2G4] at 2-5 µg for 1x10^6 cells. Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate.

  • ARG56964 anti-Liver FABP antibody [2G4] IHC-P image

    Immunohistochemistry: Paraffin embedded sections of Human colon cancer tissue stained with ARG56964 anti-Liver FABP antibody [2G4] at 1:100 for 2 hours at RT. Antigen Retrieval: Boil tissue section in 0.1M Sodium citrate buffer (pH 6.0) for 20 min.

  • ARG56964 anti-Liver FABP antibody [2G4] WB image

    Western blot: 1) 50 ng of FABP1 Recombinant protein, 2) 40 µg of HepG2 cell lysate, 3) 40 µg of Liver tissues lysate stained with ARG56964 anti-Liver FABP antibody [2G4] at 1:1000.

参考文献

Shigella flexneri Targets Human Colonic Goblet Cells by O Antigen Binding to Sialyl-Tn and Tn Antigens via Glycan-Glycan Interactions.

ICC/IF / Human

Elizabeth Ngoc Hoa Tran et al.
ACS Infect Dis.,  (2020)

publication_link

 

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