anti-Liver FABP antibody [2G4]
产品描述 | Mouse Monoclonal antibody [2G4] recognizes Liver FABP |
---|---|
反应物种 | Hu |
应用 | FACS, ICC/IF, IHC-P, WB |
宿主 | Mouse |
克隆 | Monoclonal |
克隆号 | 2G4 |
同位型 | IgG1, kappa |
靶点名称 | Liver FABP |
抗原物种 | Human |
抗原 | Recombinant fragment around aa. 1-127 of Human Liver FABP. |
偶联标记 | Un-conjugated |
別名 | FABPL; Fatty acid-binding protein, liver; Liver-type fatty acid-binding protein; L-FABP; Fatty acid-binding protein 1 |
应用建议 |
| ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 0.1M Sodium citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
形式 | Liquid |
---|---|
纯化 | Purification with Protein G. |
缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
抗菌剂 | 0.02% Sodium azide |
稳定剂 | 10% Glycerol |
浓度 | 1 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
数据库连接 | |
---|---|
基因名称 | FABP1 |
全名 | fatty acid binding protein 1, liver |
背景介绍 | This gene encodes the fatty acid binding protein found in liver. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. This protein and FABP6 (the ileal fatty acid binding protein) are also able to bind bile acids. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq, Mar 2011] |
生物功能 | Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport. [UniProt] |
预测分子量 | 14 kDa |
ARG56964 anti-Liver FABP antibody [2G4] ICC/IF image
Immunoflorescense: Hep3B cell line stained with ARG56964 anti-Liver FABP antibody [2G4] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
ARG56964 anti-Liver FABP antibody [2G4] FACS image
Flow Cytometry: Hep3B cell line stained with ARG56964 anti-Liver FABP antibody [2G4] at 2-5 µg for 1x10^6 cells. Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate.
ARG56964 anti-Liver FABP antibody [2G4] IHC-P image
Immunohistochemistry: Paraffin embedded sections of Human colon cancer tissue stained with ARG56964 anti-Liver FABP antibody [2G4] at 1:100 for 2 hours at RT. Antigen Retrieval: Boil tissue section in 0.1M Sodium citrate buffer (pH 6.0) for 20 min.
ARG56964 anti-Liver FABP antibody [2G4] WB image
Western blot: 1) 50 ng of FABP1 Recombinant protein, 2) 40 µg of HepG2 cell lysate, 3) 40 µg of Liver tissues lysate stained with ARG56964 anti-Liver FABP antibody [2G4] at 1:1000.