anti-Macrophage antibody [BA4D5]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [BA4D5] recognizes MacrophageThis antibody recognizes Porcine cells of the monocyte/macrophage lineage. Expression of the antigen is increased with maturation, with higher expression on peritoneal and alveolar macrophages.Some expression has also been observed on peripheral blood lymphocytes.The antigen recognized by clone BA4D5 has a broad tissue distribution and this antibody stains macrophages in a range of tissues, including the thymus, spleen periarteriolar lymphoid sheath (PALS), spleen red pulp and the Peyeras patches. Expression has also been reported on some non-heamatopoietic cells including endothelial cells.It is believed that clone BA4D5 may be specific for Porcine CD68, although the protein recognized by this antibody has not yet been fully characterized. The protein is expressed on the cell surface, although it is most abundantly expressed in the cytoplasm.
  • 反应物种: Pig
  • 应用: FACS, IHC-Fr, IP, WB
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: BA4D5
  • 同位型: IgG2b
  • 靶点名称: Macrophage
  • 抗原物种: Pig
  • Brand:
CAT.NO. : ARG22751
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Product Details
概述
产品描述Mouse Monoclonal antibody [BA4D5] recognizes Macrophage
This antibody recognizes Porcine cells of the monocyte/macrophage lineage. Expression of the antigen is increased with maturation, with higher expression on peritoneal and alveolar macrophages.
Some expression has also been observed on peripheral blood lymphocytes.
The antigen recognized by clone BA4D5 has a broad tissue distribution and this antibody stains macrophages in a range of tissues, including the thymus, spleen periarteriolar lymphoid sheath (PALS), spleen red pulp and the Peyeras patches. Expression has also been reported on some non-heamatopoietic cells including endothelial cells.
It is believed that clone BA4D5 may be specific for Porcine CD68, although the protein recognized by this antibody has not yet been fully characterized. The protein is expressed on the cell surface, although it is most abundantly expressed in the cytoplasm.
反应物种Pig
应用FACS, IHC-Fr, IP, WB
宿主Mouse
克隆Monoclonal
克隆号BA4D5
同位型IgG2b
靶点名称Macrophage
抗原物种Pig
抗原Porcine alveolar macrophages.
偶联标记Un-conjugated
应用说明
应用建议
应用推荐稀释比
FACS1:50 - 1:100
IHC-FrAssay-dependent
IPAssay-dependent
WBAssay-dependent (under non-reducing conditions)
应用说明WB: The antibody recognizes a 105 kDa antigen in pig macrophage lysates under non-reducing conditions.
FACS: Membrane permeabilization is required for this application. Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purification with Protein G.
缓冲液PBS and 0.09% Sodium azide.
抗菌剂0.09% Sodium azide
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
检测图片 (1)
  • ARG22751 anti-Macrophage antibody [BA4D5] FACS image

    Flow Cytometry: Pig peripheral blood monocytes stained with ARG22751 anti-Macrophage antibody [BA4D5] followed by Goat anti-Mouse IgG (FITC).

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