anti-MICA antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
CAT.NO. : ARG42662
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MICA |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MICA |
| 抗原物种 | Human |
| 抗原 | A 16 amino acid synthetic peptide within the last 50 amino acids of Human MICA. |
| 偶联标记 | Un-conjugated |
| 別名 | MHC class I polypeptide-related sequence A; PERB11.1; MIC-A |
应用说明
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | A-20 | ||||||
| 实际分子量 | ~ 46 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide. |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # Q29983 Human MHC class I polypeptide-related sequence A |
|---|---|
| 基因名称 | MICA |
| 全名 | MHC class I polypeptide-related sequence A |
| 背景介绍 | This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| 生物功能 | Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. Cytoplasm. Note=Expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Infection with human adenovirus 5 suppresses cell surface expression due to the adenoviral E3-19K protein which causes retention in the endoplasmic reticulum. [UniProt] |
| 预测分子量 | 43 kDa |
| 翻译后修饰 | N-glycosylated. Glycosylation is not essential for interaction with KLRK1/NKG2D but enhances complex formation. Proteolytically cleaved and released from the cell surface of tumor cells which impairs KLRK1/NKG2D expression and T-cell activation. [UniProt] |
检测图片 (3)
ARG42662 anti-MICA antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon tissue stained with ARG42662 anti-MICA antibody at 10 µg/ml dilution.
ARG42662 anti-MICA antibody WB image
Western blot: A-20 cell lysates stained with ARG42662 anti-MICA antibody at 0.5, 1 and 2 µg/ml dilution.
ARG42662 anti-MICA antibody IHC image
Immunohistochemistry: Human colon tissue stained with ARG42662 anti-MICA antibody at 20 µg/ml dilution.
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