anti-MLKL antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
CAT.NO. : ARG43708
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Size:
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MLKL |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MLKL |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide derived from Human MLKL. |
| 偶联标记 | Un-conjugated |
| Protein Full name | Mixed lineage kinase domain-like protein |
| 別名 | Mixed lineage kinase domain-like protein; hMLKL |
应用说明
| 应用建议 |
| ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Human esophagel cancer; THP1 | ||||||||
| 实际分子量 | ~ 54 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # Q8NB16 Human Mixed lineage kinase domain-like protein Swiss-port # Q9D2Y4 Mouse Mixed lineage kinase domain-like protein |
|---|---|
| 基因名称 | MLKL |
| 全名 | mixed lineage kinase domain-like |
| 背景介绍 | This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015] |
| 生物功能 | Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity. [UniProt] |
| 细胞定位 | Cytoplasm. Cell membrane. Note=Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30344 Necrosome Antibody Panel Related products: MLKL antibodies; MLKL Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: RIP1 activation and pathogenesis of NASH Ripoptosome & Necrosome antibody panels are launched |
| 预测分子量 | 54 kDa |
| 翻译后修饰 | Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis. It also induces homotrimerization and localization to the plasma membrane. [UniProt] |
检测图片 (2)
ARG43708 anti-MLKL antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human esophagel cancer stained with ARG43708 anti-MLKL antibody at 1:100 dilution at 4°C for overnight. Antigen retrieval: Citrate buffer (pH 6.0).
ARG43708 anti-MLKL antibody WB image
Western blot: THP1 cell lysate stained with ARG43708 anti-MLKL antibody.
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