Anti-Monomethyl-Histone H3 (Lys56) Rabbit Monoclonal Antibody [Clone RM180]; H3K56me1; Histone H3 (monomethyl K56); 31-1095-00; 31-1095-00-S
![Anti-Monomethyl-Histone H3 (Lys56) Rabbit Monoclonal Antibody [Clone RM180]; H3K56me1; Histone H3 (monomethyl K56); 31-1095-00; 31-1095-00-S](/upload/image/products/Fig-2_RM180_H3k56m1.png)
![Anti-Monomethyl-Histone H3 (Lys56) Rabbit Monoclonal Antibody [Clone RM180]; H3K56me1; Histone H3 (monomethyl K56); 31-1095-00; 31-1095-00-S](/upload/image/products/Fig1_RM180_H3K56m1_e.png)
Key features and details
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CAT.NO. : 31-1095-00
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Product Details
Product Details
| Product Name | Anti-Monomethyl-Histone H3 (Lys56) Rabbit Monoclonal Antibody, Clone RM180 |
| Product Description | Rabbit monoclonal to Monomethylated Histone H3 Lysine 56, H3K56me1, Histone H3 (monomethyl K56) |
| Catalog No. | 31-1095-00 |
| Clone Name | RM180 |
| Product Data Sheet | Anti-Monomethyl-Histone H3 (Lys56) Rabbit Monoclonal Antibody Data Sheet RM180 |
| Specificity | RM180 reacts to Histone H3 monomethylated at Lysine 56 (K56me1). No cross reactivity with nonmodified Lysine 56 or other methylations in histone H3. |
| Immunogen | A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys56). |
| Application | Western Blot (WB) ELISA Multiplex |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 μg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 1 μg/mL – 2 μg/mL; ELISA: 0.2 μg/mL - 1 μg/mL; Multiplex: 0.1 μg/mL – 0.5 μg/mL. |
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Product Images
 | RM180 specifically reacts to Histone H3 monomethylated at Lysine 56 (K56me1). No cross reactivity with nonmodified Lysine 56 (K56Ctrl), or other methylations in histone H3. |
 | Western Blot of acid extracts of HeLa cells (1) and recombinant histone H3.3 (2), using RM180 at 1 μg/mL, showed a band of histone H3 monomethylated at Lysine 56 (K56me1) in HeLa cells. |
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