Anti-Mouse IgG2b Rabbit Monoclonal Antibody [Clone RM108]; 31-1004-00
![Anti-Mouse IgG2b Rabbit Monoclonal Antibody [Clone RM108]; 31-1004-00](/upload/image/products/RM108_WB.png)
![Anti-Mouse IgG2b Rabbit Monoclonal Antibody [Clone RM108]; 31-1004-00](/upload/image/products/Anri-mouse-IgG2b-2.jpg)
![Anti-Mouse IgG2b Rabbit Monoclonal Antibody [Clone RM108]; 31-1004-00](/upload/image/products/Anri-mouse-IgG2b-3.jpg)
Key features and details
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Product Details
| Product Name | Anti-Mouse IgG2b Rabbit Monoclonal Antibody, Clone RM108 |
| Product Description | Rabbit monoclonal to Mouse IgG2b |
| Catalog No. | 31-1004-00 |
| Clone Name | RM108 |
| Product Data Sheet | Anti-Mouse IgG2b Rabbit Monoclonal Antibody Data Sheet RM108 |
| Specificity | RM108 reacts to the Fc region of mouse IgG2b. No cross reactivity with mouse IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The Fc region of RM108 has been engineered to eliminate Fc receptor binding. |
| Immunogen | Mouse IgG2b |
| Application | ELISA Western Blot (WB) (stronger on nonreduced) |
| Species Reactivity | Mouse |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | ELISA: 0.005 µg/mL – 0.2 µg/mL; Western Blot (WB): 0.1 µg/mL - 0.5 µg/mL. |
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 | Western blot of nonreduced (-) and reduced (+) mouse IgG2b (20 ng/lane), using 0.2 µg/mL of RevMAb clone RM108. This antibody reacts to nonreduced IgG2b (~150 kDa) much stronger than the reduced γ2b form (~50 kDa). |
 | ELISA of mouse immunoglobulins shows RM108 reacts to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM108 was used as the primary antibody. An alkaline phosph-atase conjugated anti-rabbit IgG as the secondary antibody. |
 | A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. A serial dilution of RM108 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
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