Anti-Mouse Immunoglobulin Lambda Light Chain Rabbit Monoclonal Antibody [Clone RM110]; 31-1006-00

Key features and details

  • Uniprot: P01843
  • Gene ID: 110785
  • Host Species: Rabbit
  • Species Reactivity: Mouse
  • Concentration: 1 mg/mL
  • Brand:
CAT.NO. : 31-1006-00
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Product Details
Product Details
Product NameAnti-Mouse Immunoglobulin Lambda Light Chain Rabbit Monoclonal Antibody, Clone RM110
Product DescriptionRabbit monoclonal to Mouse Ig Lambda Light Chain
Catalog No.31-1006-00
Clone NameRM110
Product Data SheetAnti-Mouse Ig Lambda Rabbit Monoclonal Antibody Data Sheet RM110
SpecificityRM110 reacts to the lambda light chain of mouse immunoglobulins. No cross reactivity with the kappa light chain, human IgG, rat IgG, or goat IgG.
The Fc region of RM110 has been engineered to eliminate Fc receptor binding.
ImmunogenMouse IgM lambda
ApplicationWestern Blot (WB) (nonreduced)
ELISA
Species ReactivityMouse
ConjugateNone
Intended UseResearch Use Only
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Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100 µg
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 0.005 µg/mL - 0.2 µg/mL
Western Blot (WB): 0.1 µg/mL - 0.5 µg/mL
Keywords
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Product Images
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Western blot of nonreduced (-) and reduced (+) mouse IgG1κ and IgG1λ (20 ng/lane), using 0.2 µg/mL of RevMAb Clone RM110. This antibody reacts to nonreduced IgG1λ.

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ELISA of mouse immunoglobulins shows RM110 reacts to the lambda light chain of mouse immuno- globulins. No cross reactivity with the kappa light chain, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immuno-globulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM110 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

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A titer ELISA of mouse IgG1λ. The plate was coated with different amounts of mouse IgG1λ. A serial dilution of RM110 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit lgG as the secondary antibody.

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