anti-MSH2 antibody [SQab20226]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [SQab20226] recognizes MSH2
  • 反应物种: Hu
  • 应用: IHC-P
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: SQab20226
  • 同位型: IgG
  • 靶点名称: MSH2
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG66817
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Product Details
概述
产品描述Mouse Monoclonal antibody [SQab20226] recognizes MSH2
反应物种Hu
应用IHC-P
宿主Mouse
克隆Monoclonal
克隆号SQab20226
同位型IgG
靶点名称MSH2
抗原物种Human
抗原Recombinant fragment around aa. 327-427 of Human MSH2 protein.
偶联标记Un-conjugated
別名DNA mismatch repair protein Msh2; COCA1; HNPCC1; FCC1; LCFS2; MutS protein homolog 2; hMSH2; HNPCC
应用说明
应用建议
应用推荐稀释比
IHC-P1:100 - 1:200
应用说明IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C - 25°C) for 30 minutes.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purification with Protein G.
缓冲液PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂0.01% Sodium azide
稳定剂40% Glycerol and 0.05% BSA
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 4436 Human MSH2

Swiss-port # P43246 Human DNA mismatch repair protein Msh2

基因名称MSH2
全名mutS homolog 2
背景介绍This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
生物功能Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containing DNA strand (PubMed:26300262). ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis. [UniProt]
细胞定位Nucleus. [UniProt]
预测分子量105 kDa
翻译后修饰Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway. [UniProt]
检测图片 (1)
  • ARG66817 anti-MSH2 antibody [SQab20226] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human colon carcinoma tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66817 anti-MSH2 antibody [SQab20226].

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