anti-MUC16 / CA125 antibody [X75]
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![anti-MUC16 / CA125 antibody [X75]](/upload/image/products/ARG10156-ARG10351_ELISA_200608.jpg)
![anti-MUC16 / CA125 antibody [X75]](/upload/image/products/ARG10351-ARG10156_ELISA_200608.jpg)
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
Product Details
| 产品描述 | Mouse Monoclonal antibody [X75] recognizes MUC16 / CA125 |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, FACS, ICC/IF, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | X75 |
| 同位型 | IgG1 |
| 靶点名称 | MUC16 / CA125 |
| 抗原物种 | Human |
| 抗原 | MUC16 / CA125 antigen purified from human ovarian carcinoma (MW > 1 MDa). |
| 抗原表位 | Epitope specificity group B (ISOBM classification) similar to M-11. |
| 偶联标记 | Un-conjugated |
| 別名 | Mucin-16; MUC-16; Ovarian carcinoma antigen CA125; CA125; Ovarian cancer-related tumor marker CA125; CA-125 |
| 应用建议 |
| ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | Sandwich ELISA (Capture antibody - Detection antibody): ARG10156 - ARG10351 ARG10351 - ARG10156 ARG10534 - ARG10156 IHC-P: Antigen Retrieval: Heat mediation was performed. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Ion exchange chromatography. |
| 缓冲液 | 10 mM Tris (pH 7.5), 0.15 M NaCl and 0.05% Sodium azide. |
| 抗菌剂 | 0.05% Sodium azide |
| 浓度 | 1.0-2.0 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | MUC16 |
| 全名 | mucin 16, cell surface associated |
| 生物功能 | Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. [UniProt] |
| 研究领域 | Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody |
| 预测分子量 | 1519 kDa |
| 翻译后修饰 | Heavily O-glycosylated; expresses both type 1 and type 2 core glycans. Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans. May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space. May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts. |
ARG10156 anti-MUC16 / CA125 antibody [X75] IHC-P image
Immunohistochemistry: Paraffin-embedded Human ovarian cancer tissue. Antigen Retrieval: Heat mediation was performed. The tissue section was stained with ARG10156 anti-MUC16 / CA125 antibody [X75] at 1:200 dilution.
ARG10156 anti-MUC16 / CA125 antibody [X75] standard curve image
ELISA: Analysis of Human CA125 expression using ARG10156 anti-MUC16 / CA125 antibody [X75] as a capture antibody and biotinylated ARG10351 anti-MUC16 / CA125 antibody [X325] as a detection antibody with purified Human CA125 as antigen to generate the standard curve. Detection is by HRP conjugated streptavidin and substrate. Plasma (green) sample at 1:2 is shown.
ARG10156 anti-MUC16 / CA125 antibody [X75] standard curve image
ELISA: Analysis of Human CA125 expression using ARG10351 anti-MUC16 / CA125 antibody [X325] as a capture antibody and biotinylated ARG10156 anti-MUC16 / CA125 antibody [X75] as a detection antibody with purified Human CA125 as antigen to generate the standard curve. Detection is by HRP conjugated streptavidin and substrate. Plasma (green) sample at 1:2 is shown.
克隆号文献
New construction of an animal model for the orthotopic transplantation of an ovarian tumor.
IHC-P, FACS /
The use of ovarian cancer cells from patients undergoing surgery to generate primary cultures capable of undergoing functional analysis.
ICC/IF / Human
Novel treatment option for MUC16-positive malignancies with the targeted TRAIL-based fusion protein Meso-TR3.
FACS / Human
Mucin 16 is a functional selectin ligand on pancreatic cancer cells.
IP / Human
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