anti-MURF3 / TRIM54 antibody
CAT.NO. : ARG63294
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概述
产品描述 | Goat Polyclonal antibody recognizes MURF3 / TRIM54 |
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反应物种 | Hu |
应用 | IHC-P, WB |
宿主 | Goat |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | MURF3 / TRIM54 |
抗原物种 | Human |
抗原 | C-GSAGPEEERPDGP |
偶联标记 | Un-conjugated |
別名 | Muscle-specific RING finger protein; Tripartite motif-containing protein 54; muRF3; MuRF-3; MURF-3; MURF; MuRF3; MuRF; Muscle-specific RING finger protein 3; RNF30; RING finger protein 30 |
应用说明
应用建议 |
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应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
形式 | Liquid |
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纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
抗菌剂 | 0.02% Sodium azide |
稳定剂 | 0.5% BSA |
浓度 | 0.5 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
数据库连接 | Swiss-port # Q9BYV2 Human Tripartite motif-containing protein 54 |
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背景介绍 | The protein encoded by this gene contains a RING finger motif and is highly similar to the ring finger proteins RNF28/MURF1 and RNF29/MURF2. In vitro studies demonstrated that this protein, RNF28, and RNF29 form heterodimers, which may be important for the regulation of titin kinase and microtubule-dependent signal pathways in striated muscles. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody |
预测分子量 | 40 kDa |
检测图片 (2)
ARG63294 anti-MURF3 / TRIM54 antibody WB image
Western Blot: human muscle lysate (RIPA buffer, 35ug total protein per lane) stained with ARG63294 anti-MURF3 / TRIM54 antibody at 1ug/ml dilution.
ARG63294 anti-MURF3 / TRIM54 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human skeletal muscle tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63294 anti-MURF3 / TRIM54 antibody at 3.75 µg/ml dilution followed by AP-staining.
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