anti-Myelin Basic Protein antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
-
Brand:
Product Details
| 产品描述 | Chicken Polyclonal antibody recognizes Myelin Basic Protein (MBP) |
|---|---|
| 反应物种 | Hu, Ms, Rat, Bov |
| 预测物种 | Mamm |
| 应用 | ICC/IF, IHC-Fr, WB |
| 宿主 | Chicken |
| 克隆 | Polyclonal |
| 同位型 | IgY |
| 靶点名称 | Myelin Basic Protein |
| 抗原物种 | Rat |
| 抗原 | Three peptide sequences conserved in higher vertebrate MBP proteins |
| 偶联标记 | Un-conjugated |
| 別名 | Myelin A1 protein; MBP; Myelin membrane encephalitogenic protein; Myelin basic protein |
| 应用建议 |
| ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | Specific for the ~18k & 22k myelin basic protein in Western blots of Rat brain homogenate. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Total IgY fraction |
| 缓冲液 | Total IgY fraction in PBS and 5 mM Sodium azide |
| 抗菌剂 | 5 mM Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | MBP |
| 全名 | myelin basic protein |
| 背景介绍 | Myelin Basic Protein (MBP) is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago (Eylar et al., 1971). The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells (Marty et al., 2002). In the central nervous system there are there different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5, and 17.2 kDa. Since the two lower molecular weight forms are very close in molecular size, MBP antibodies typically show two bands on Western blots, one at about 22kDa and another at about 18kDa. |
| 研究领域 | Controls and Markers antibody; Neuroscience antibody |
| 预测分子量 | 33 kDa |
| 翻译后修饰 | Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic. The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6). Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated. Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1. |
ARG52344 anti-Myelin Basic Protein (MBP) antibody ICC/IF image
Immunofluorescence: Rat mixed neuron/glial cultures stained with ARG52344 anti-Myelin Basic Protein (MBP) antibody (green). Blue is a DNA stain. Note that the Myelin Basic Protein antibody stains an oligodendrocyte and some membrane shed from this cell. Other cells in the field include neurons, astrocytes, microglia and fibroblasts, all of which are completely negative.
ARG52344 anti-Myelin Basic Protein (MBP) antibody IHC-Fr image
Immunohistochemistry: Frozen section of Rat brain cerebellum tissue stained with ARG52344 anti-Myelin Basic Protein (MBP) antibody (green) at 1:5000 dilution, and co-stained with ARG10732 anti-Neurofilament NF-L antibody (red) at 1:5000 dilution.
The MBP antibody stains oligodendrocytes and myelin sheathes around axons, while the NF-L antibody labels dendrites and axons of neuronal cells.
ARG52344 anti-Myelin Basic Protein (MBP) antibody WB image
Western blot: Rat cortex homogenate showing specific immunolabeling of the ~18 and 22k MBP protein stained with ARG52344 anti-Myelin Basic Protein (MBP) antibody.
New Products
