anti-N Cadherin antibody [8C11]
![anti-N Cadherin antibody [8C11]](/upload/image/products/ARG57525_WB_HuBrain_210_205.jpg)
![anti-N Cadherin antibody [8C11]](/upload/image/products/ARG57525_IHC_MsHeart.jpg)
![anti-N Cadherin antibody [8C11]](/upload/image/products/ARG57525_WB_HuBrain.jpg)
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG57525
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [8C11] recognizes N Cadherin |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 8C11 |
| 同位型 | IgG1, kappa |
| 靶点名称 | N Cadherin |
| 抗原物种 | Human |
| 抗原 | Partial recombinant protein of Human N-Cadherin (extracellular domain). |
| 偶联标记 | Un-conjugated |
| 別名 | Neural cadherin; N-cadherin; CDw325; CDHN; CD antigen CD325; NCAD; Cadherin-2; CD325 |
应用说明
| 应用建议 |
| ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS, 0.05% Sodium azide and 0.1 mg/ml BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 0.1 mg/ml BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CDH2 |
| 全名 | cadherin 2, type 1, N-cadherin (neuronal) |
| 背景介绍 | N Cadherin is a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015] |
| 生物功能 | N Cadherin is a calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein Cell membrane; sarcolemma Cell junction Cell surface Note: Colocalizes with TMEM65 at the intercalated disk in cardiomyocytes. Colocalizes with OBSCN at the intercalated disk and at sarcolemma in cardiomyocytes. [UniProt] |
| 研究领域 | EMT Study antibody; Mesenchymal Markers antibody |
| 预测分子量 | ~ 100 kDa (unmodified), 125-140 kDa (modified). |
| 翻译后修饰 | Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity). May be phosphorylated by OBSCN. [UniProt] |
检测图片 (2)
ARG57525 anti-N Cadherin antibody [8C11] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Mouse heart tissue stained with ARG57525 anti-N Cadherin antibody [8C11]. Antigen Retrieval: Boil tissue section in 10mM Tris with 1mM EDTA (pH 9.0) for 10-20 min followed by cooling at RT for 20 min.
ARG57525 anti-N Cadherin antibody [8C11] WB image
Western blot: Human brain lysate stained with ARG57525 anti-N Cadherin antibody [8C11].
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