anti-NDUFS1 antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes NDUFS1
  • 反应物种: Hu, Ms
  • 预测物种: Cow, Rat, Dog
  • 应用: IHC-P, WB
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: NDUFS1
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG65261
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes NDUFS1
反应物种Hu, Ms
预测物种Cow, Rat, Dog
应用IHC-P, WB
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称NDUFS1
抗原物种Human
抗原C-TEKSATYVNTEGR
偶联标记Un-conjugated
別名CI-75k; EC 1.6.5.3; NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; CI-75Kd; EC 1.6.99.3; PRO1304; Complex I-75kD; CI-75kD
应用说明
应用建议
应用推荐稀释比
IHC-P5 µg/ml
WB0.3 - 1 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 227197 Mouse NDUFS1

GeneID: 4719 Human NDUFS1

Swiss-port # P28331 Human NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

Swiss-port # Q91VD9 Mouse NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

背景介绍The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
研究领域Cancer antibody; Controls and Markers antibody; Metabolism antibody; Neuroscience antibody
预测分子量79 kDa
检测图片 (2)
  • ARG65261 anti-NDUFS1 antibody WB image

    Western Blot: Human Heart lysate (35 µg protein in RIPA buffer) stained with ARG65261 anti-NDUFS1 antibody at 0.3 µg/ml dilution.

  • ARG65261 anti-NDUFS1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human heart tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65261 anti-NDUFS1 antibody at 5 µg/ml dilution followed by AP-staining.

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