anti-Neurofilament NF-L antibody [1B11]
![anti-Neurofilament NF-L antibody [1B11]](/upload/image/products/ARG11122_WB_1_210_205.jpg)
![anti-Neurofilament NF-L antibody [1B11]](/upload/image/products/ARG11122_IHC-Fr_1.jpg)
![anti-Neurofilament NF-L antibody [1B11]](/upload/image/products/ARG11122_WB_1.jpg)
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
Product Details
| 产品描述 | Mouse Monoclonal antibody [1B11] recognizes Neurofilament NF-L |
|---|---|
| 反应物种 | Hu, Ms, Rat, Cow, Hrs, Pig |
| 应用 | ICC/IF, IHC-Fr, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1B11 |
| 同位型 | IgG1 |
| 靶点名称 | Neurofilament NF-L |
| 抗原物种 | Pig |
| 抗原 | Pig Neurofilament NF-L. |
| 抗原表位 | Within the C terminal segment of alpha-helical region, a highly conserved region known as Coil II, corresponding to aa. 256-400 of the human protein. |
| 偶联标记 | Un-conjugated |
| 別名 | Neurofilament triplet L protein; 68 kDa neurofilament protein; CMT1F; NF68; NFL; CMT2E; Neurofilament light polypeptide; NF-L; PPP1R110 |
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | ~ 62 kDa |
| 形式 | Liquid |
|---|---|
| 纯化 | Purified |
| 缓冲液 | PBS, 5 mM Sodium azide and 50% Glycerol. |
| 抗菌剂 | 5 mM Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
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| 基因名称 | NEFL |
| 全名 | neurofilament, light polypeptide |
| 背景介绍 | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008] |
| 生物功能 | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. [UniProt] |
| 预测分子量 | 62 kDa |
| 翻译后修饰 | O-glycosylated. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. Ubiquitinated in the presence of TRIM2 and UBE2D1. [UniProt] |
ARG11122 anti-Neurofilament NF-L antibody [1B11] IHC-Fr image
Immunohistochemistry: Frozen section of cow cerebellum tissue stained with ARG11122 anti-Neurofilament NF-L antibody [1B11] (green) at 1:2000 dilution, and co-stained with ARG10688 anti-Visinin like 1 antibody (red) at 1:2000 dilution. Hoechst (blue) for nuclear staining. (Sample preparation: Small section of cow cerebellum was fixed in 4% paraformaldehyde for 3 days, cut to 45 µM, and free-floating sections were stained with the above antibodies.).
ARG11122 anti-Neurofilament NF-L antibody [1B11] WB image
Western blot: Rat brain, Mouse brain and Cow cerebellum lysates stained with ARG11122 anti-Neurofilament NF-L antibody [1B11] (green) at 1:20000 dilution.
Strong band at about 68-70 kDa corresponds to NF-L protein, with the cow protein appearing slightly larger in molecular size as expected. Low molecular weight bands detected in cow brain sample are likely post mortem proteolytic forms of NF-L.
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