anti-NFIL3 antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes NFIL3
  • 反应物种: Hu
  • 预测物种: Ms, Rat
  • 应用: FACS, WB
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: NFIL3
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG63152
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes NFIL3
反应物种Hu
预测物种Ms, Rat
应用FACS, WB
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称NFIL3
抗原物种Human
抗原C-RLIATQPISASDSG
偶联标记Un-conjugated
別名Nuclear factor interleukin-3-regulated protein; NF-IL3A; E4BP4; IL3BP1; Transcriptional activator NF-IL3A; NFIL3A; Interleukin-3 promoter transcriptional activator; E4 promoter-binding protein 4; Interleukin-3-binding protein 1
应用说明
应用建议
应用推荐稀释比
FACS10 µg/ml
WB0.5 - 2 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 4783 Human NFIL3

Swiss-port # Q16649 Human Nuclear factor interleukin-3-regulated protein

背景介绍Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells, natural killer (NK) cells, and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors, such as NFIL3, that bind to regulatory regions of the gene, usually upstream of the transcription start site (Zhang et al., 1995 [PubMed 7565758]).[supplied by OMIM, Feb 2009]
研究领域Gene Regulation antibody; Immune System antibody
预测分子量51 kDa
检测图片 (2)
  • ARG63152 anti-NFIL3 antibody WB image

    Western Blot: HepG2 lysate (RIPA buffer, 20 µg total protein per lane) stained with ARG63152 anti-NFIL3 antibody at 0.5 µg/ml dilution.

  • ARG63152 anti-NFIL3 antibody FACS image

    Flow Cytometry: Paraformaldehyde-fixed Human peripheral blood mononuclear cells permeabilized with 0.5% Triton. Cells were stained with ARG63152 anti-NFIL3 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).

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