anti-NSE / Neuron Specific Enolase antibody

Key features and details

  • 产品描述: Chicken Polyclonal antibody recognizes NSE / Neuron Specific Enolase
  • 反应物种: Hu, Ms, Rat, Cow
  • 应用: IHC-Fr, WB
  • 宿主: Chicken
  • 克隆: Polyclonal
  • 同位型: IgY
  • 靶点名称: NSE / Neuron Specific Enolase
  • 抗原物种: Human
  • 抗原: Recombinant full-length Human NSE / Neuron Specific Enolase.
  • Brand:
CAT.NO. : ARG11115
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Product Details
概述
产品描述Chicken Polyclonal antibody recognizes NSE / Neuron Specific Enolase
反应物种Hu, Ms, Rat, Cow
应用IHC-Fr, WB
宿主Chicken
克隆Polyclonal
同位型IgY
靶点名称NSE / Neuron Specific Enolase
抗原物种Human
抗原Recombinant full-length Human NSE / Neuron Specific Enolase.
偶联标记Un-conjugated
別名Neural enolase; NSE; Enolase 2; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; HEL-S-279; Neuron-specific enolase; EC 4.2.1.11
应用说明
应用建议
应用推荐稀释比
IHC-Fr1:500 - 1:1000
WB1:5000 - 1:10000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量~ 45 kDa
属性
形式Liquid
缓冲液PBS and 5 mM Sodium azide.
抗菌剂5 mM Sodium azide
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 13807 Mouse ENO2

GeneID: 2026 Human ENO2

GeneID: 24334 Rat ENO2

基因名称ENO2
全名enolase 2 (gamma, neuronal)
背景介绍This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]
生物功能Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity). [UniProt]
细胞定位Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. [UniProt]
预测分子量47 kDa
检测图片 (2)
  • ARG11115 anti-NSE / Neuron Specific Enolase antibody IHC-Fr image

    Immunohistochemistry: Frozen section of adult mouse cerebellar dentate nucleus stained with ARG11115 anti-NSE / Neuron Specific Enolase antibody (red) at 1:3000 dilution. The section was co-stained with anti-GFAP antibody (green) at 1:5000 dilution. Hoechst (blue) for nuclear staining. (Sample preparation: Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies.).

  • ARG11115 anti-NSE / Neuron Specific Enolase antibody WB image

    Western blot: Rat brain, Mouse brain, Cow brain and SH-SY5Y cell lysates stained with ARG11115 anti-NSE / Neuron Specific Enolase antibody at 1:5000 dilution.

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