anti-OGT antibody
产品描述 | Goat Polyclonal antibody recognizes OGT |
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反应物种 | Hu, Rat |
预测物种 | Ms, Cow, Dog |
应用 | FACS, ICC/IF, IHC-P, WB |
特异性 | This antibody is expected to recognise both reported isoforms (NP_858058.1 and NP_858059.1 |
宿主 | Goat |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | OGT |
抗原物种 | Human |
抗原 | C-YEHPKDLKLSDGR |
偶联标记 | Un-conjugated |
別名 | UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; O-GLCNAC; EC 2.4.1.255; OGT; HRNT1; HINCUT-1; O-linked N-acetylglucosamine transferase 110 kDa subunit; O-GlcNAc transferase subunit p110 |
应用建议 |
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应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
形式 | Liquid |
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纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
抗菌剂 | 0.02% Sodium azide |
稳定剂 | 0.5% BSA |
浓度 | 0.5 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
数据库连接 | Swiss-port # O15294 Human UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa s Swiss-port # P56558 Rat UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa sub |
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背景介绍 | This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2009] |
研究领域 | Neuroscience antibody; Signaling Transduction antibody |
预测分子量 | 117 kDa |
翻译后修饰 | Ubiquitinated, leading to its proteasomal degradation. Phosphorylation on Ser-3 or Ser-4 by GSK3-beta positively regulates its activity. |
ARG64143 anti-OGT antibody WB image
Western Blot: Rat Pancreas lysate (35 µg protein in RIPA buffer) stained with ARG64143 anti-OGT antibody at 0.05 µg/ml dilution.
ARG64143 anti-OGT antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U251 cells permeabilized with 0.15% Triton. Cells were stained with ARG64143 anti-OGT antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
ARG64143 anti-OGT antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64143 anti-OGT antibody at 5 µg/ml dilution followed by AP-staining.
ARG64143 anti-OGT antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HEK293 cells permeabilized with 0.5% Triton. Cells were stained with ARG64143 anti-OGT antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
ARG64143 anti-OGT antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG64143 anti-OGT antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Phalloidin (red) for Actin filaments staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
ARG64143 anti-OGT antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG64143 anti-OGT antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Phalloidin (red) for Actin filaments staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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