anti-OGT antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes OGT
  • 反应物种: Hu, Rat
  • 预测物种: Ms, Cow, Dog
  • 应用: FACS, ICC/IF, IHC-P, WB
  • 特异性: This antibody is expected to recognise both reported isoforms (NP_858058.1 and NP_858059.1
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: OGT
  • Brand:
CAT.NO. : ARG64143
US$ Please choose
US$ Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
概述
产品描述Goat Polyclonal antibody recognizes OGT
反应物种Hu, Rat
预测物种Ms, Cow, Dog
应用FACS, ICC/IF, IHC-P, WB
特异性This antibody is expected to recognise both reported isoforms (NP_858058.1 and NP_858059.1
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称OGT
抗原物种Human
抗原C-YEHPKDLKLSDGR
偶联标记Un-conjugated
別名UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; O-GLCNAC; EC 2.4.1.255; OGT; HRNT1; HINCUT-1; O-linked N-acetylglucosamine transferase 110 kDa subunit; O-GlcNAc transferase subunit p110
应用说明
应用建议
应用推荐稀释比
FACS10 µg/ml
ICC/IF10 µg/ml
IHC-P5 µg/ml
WB0.05 - 0.2 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 26295 Rat OGT

GeneID: 8473 Human OGT

Swiss-port # O15294 Human UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa s

Swiss-port # P56558 Rat UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa sub

背景介绍This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
研究领域Neuroscience antibody; Signaling Transduction antibody
预测分子量117 kDa
翻译后修饰Ubiquitinated, leading to its proteasomal degradation.
Phosphorylation on Ser-3 or Ser-4 by GSK3-beta positively regulates its activity.
检测图片 (6)
  • ARG64143 anti-OGT antibody WB image

    Western Blot: Rat Pancreas lysate (35 µg protein in RIPA buffer) stained with ARG64143 anti-OGT antibody at 0.05 µg/ml dilution.

  • ARG64143 anti-OGT antibody ICC/IF image

    Immunofluorescence: Paraformaldehyde fixed U251 cells permeabilized with 0.15% Triton. Cells were stained with ARG64143 anti-OGT antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.

  • ARG64143 anti-OGT antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64143 anti-OGT antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG64143 anti-OGT antibody FACS image

    Flow Cytometry: Paraformaldehyde-fixed HEK293 cells permeabilized with 0.5% Triton. Cells were stained with ARG64143 anti-OGT antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).

  • ARG64143 anti-OGT antibody ICC/IF image

    Immunofluorescence: Paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG64143 anti-OGT antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Phalloidin (red) for Actin filaments staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.

  • ARG64143 anti-OGT antibody ICC/IF image

    Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG64143 anti-OGT antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Phalloidin (red) for Actin filaments staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.

New Products
Get in touch with AREX
Name:*
Tel/Phone:*
Company:*
Email:*
Inquiry:
Captcha*
Submitting your email information means that you are willing to receive email information from AREX regarding technology, applications, products, and events. By clicking on the 'unsubscribe' button in the email or by contacting info@arexbio.com You can unsubscribe at any time by sending an email. Regarding your data usage information, please refer to our privacy policy.
© AREX 2024. All Rights Reserved. | 浙ICP备2024113709号-3 Sitemap