anti-PAX8 antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes PAX8
  • 反应物种: Hu
  • 预测物种: Ms, Rat
  • 应用: IHC-P, WB
  • 特异性: This antibody is expected to recognise all five human isoforms (as represented by NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1)
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: PAX8
  • Brand:
CAT.NO. : ARG63488
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes PAX8
反应物种Hu
预测物种Ms, Rat
应用IHC-P, WB
特异性This antibody is expected to recognise all five human isoforms (as represented by NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1)
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称PAX8
抗原物种Human
抗原PHNSIRSGHGGLN-C
偶联标记Un-conjugated
別名Paired box protein Pax-8
应用说明
应用建议
应用推荐稀释比
IHC-P5 µg/ml
WB1 - 2 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 7849 Human PAX8

Swiss-port # Q06710 Human Paired box protein Pax-8

背景介绍This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010]
研究领域Gene Regulation antibody; Neuroscience antibody
预测分子量48 kDa
检测图片 (4)
  • ARG63488 anti-PAX8 antibody WB image

    Western Blot: Human Kidney lysate (35 µg protein in RIPA buffer) stained with ARG63488 anti-PAX8 antibody at 1.5 µg/ml dilution.

  • ARG63488 anti-PAX8 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human kidney cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63488 anti-PAX8 antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG63488 anti-PAX8 antibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Human Thyroid Gland. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63488 anti-PAX8 antibody at 2 µg/ml dilution followed by HRP-staining.

  • ARG63488 anti-PAX8 antibody WB image

    Western blot: 35 µg of Human kidney (A) and HeLa cell (B) lysates (in RIPA buffer) stained with ARG63488 anti-PAX8 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.

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