anti-Peptide YY antibody
产品描述 | Rabbit Polyclonal antibody recognizes Peptide YY |
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反应物种 | Ms, Rat |
应用 | IHC-P, WB |
宿主 | Rabbit |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | Peptide YY |
抗原物种 | Mouse |
抗原 | Synthetic peptide corresponding to aa. 29-64 of Mouse Peptide YY (YPAKPEAPGEDASPEELSRYYASLRHYLNLVTRQRY). |
偶联标记 | Un-conjugated |
別名 | Peptide tyrosine tyrosine; PYY; PYY-I; PYY1; Peptide YY; PYY-II; 3-36 |
应用建议 |
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应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
形式 | Liquid |
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纯化 | Affinity purification with immunogen. |
缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
抗菌剂 | 0.05% Sodium azide |
稳定剂 | 5% BSA |
浓度 | 0.5 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
数据库连接 | |
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基因名称 | PYY |
全名 | peptide YY |
背景介绍 | The protein encoded by this gene is proteolytically processed to release a peptide that inhibits pancreatic secretion and mobility in the gut. Rare variations in this gene could increase susceptibility to obesity. [provided by RefSeq, Jul 2010] |
生物功能 | This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility. [UniProt] |
细胞定位 | Secreted. [UniProt] |
预测分子量 | 11 kDa |
翻译后修饰 | The peptide YY form is cleaved at Pro-30 by the prolyl endopeptidase FAP (seprase) activity (in vitro) to generate peptide YY(3-36). [UniProt] |
ARG40991 anti-Peptide YY antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40991 anti-Peptide YY antibody at 1 µg/ml dilution, overnight at 4°C.
ARG40991 anti-Peptide YY antibody WB image
Western blot: 50 µg of samples under reducing conditions. Mouse spleen and Mouse liver lysates stained with ARG40991 anti-Peptide YY antibody at 0.5 µg/ml, overnight at 4°C.
ARG40991 anti-Peptide YY antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40991 anti-Peptide YY antibody at 1 µg/ml dilution, overnight at 4°C.
ARG40991 anti-Peptide YY antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse pancreas tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40991 anti-Peptide YY antibody at 1 µg/ml dilution, overnight at 4°C.
ARG40991 anti-Peptide YY antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40991 anti-Peptide YY antibody at 1 µg/ml dilution, overnight at 4°C.
ARG40991 anti-Peptide YY antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat pancreas tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40991 anti-Peptide YY antibody at 1 µg/ml dilution, overnight at 4°C.