anti-Perforin antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
CAT.NO. : ARG40793
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Perforin |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Perforin |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 280-555 of Human Perforin (NP_001076585.1). |
| 偶联标记 | Un-conjugated |
| 別名 | FLH2; P1; PFN1; Cytolysin; HPLH2; PFP; Lymphocyte pore-forming protein; Perforin-1 |
应用说明
| 应用建议 |
| ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Mouse kidney | ||||||||
| 实际分子量 | 68 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PRF1 |
| 全名 | perforin 1 (pore forming protein) |
| 背景介绍 | Perforin with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017] |
| 生物功能 | Perforin plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. [UniProt] |
| 细胞定位 | Cytoplasmic granule lumen. Secreted. Cell membrane. Endosome lumen. Note=Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes. [UniProt] |
| 产品亮点 | Related products: Perforin antibodies; Perforin ELISA Kits; Anti-Rabbit IgG secondary antibodies; Related news: Examining CTL/NK-mediated cytotoxicity by ELISA |
| 预测分子量 | 61 kDa |
| 翻译后修饰 | N-glycosylated. [UniProt] |
检测图片 (3)
ARG40793 anti-Perforin antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG40793 anti-Perforin antibody.
ARG40793 anti-Perforin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse spleen stained with ARG40793 anti-Perforin antibody at 1:100 dilution.
ARG40793 anti-Perforin antibody WB image
Western blot: 25 µg of Mouse kidney lysate stained with ARG40793 anti-Perforin antibody at 1:1000 dilution.
参考文献
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IHC-P, WB / Human, Mouse
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