anti-Phosphothreonine antibody [RM102]

产品描述: Rabbit Monoclonal antibody [RM102] recognizes Phosphothreonine
反应物种: Other
应用: ICC/IF, IP, WB
特异性: This antibody reacts to threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated threonine, phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides.
宿主: Rabbit
克隆: Monoclonal
克隆号: RM102
同位型: IgG
靶点名称: Phosphothreonine

CAT.NO. : ARG57186

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Size:

50 μg

Product Details

概述

产品描述	Rabbit Monoclonal antibody [RM102] recognizes Phosphothreonine
反应物种	Other
应用	ICC/IF, IP, WB
特异性	This antibody reacts to threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated threonine, phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides.
宿主	Rabbit
克隆	Monoclonal
克隆号	RM102
同位型	IgG
靶点名称	Phosphothreonine
抗原物种	Others
抗原	Mixture of phosphothreonine-BSA conjugate and a phosphothreonine containing peptide.
偶联标记	Un-conjugated

应用说明

应用建议

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein A.
缓冲液	PBS, 0.09% Sodium azide, 50% Glycerol and 1% BSA.
抗菌剂	0.09% Sodium azide
稳定剂	50% Glycerol and 1% BSA
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

检测图片 (4)

ARG57186 anti-Phosphothreonine antibody [RM102] ICC/IF image
Immunofluorescence: Serum-starved A431 cells nontreated or treated with Calyculin A/Okadaic Acid, stained with ARG57186 anti-Phosphothreonine antibody [RM102] at 1:500 dilution (followed by PE-conjugated secondary antibody, red) and DAPI (blue).
ARG57186 anti-Phosphothreonine antibody [RM102] WB image
Western blot: serum-starved A431 cells non-treated or treated with Calyculin A / Okadaic Acid, stained with ARG57186 anti-Phosphothreonine antibody [RM102] at 1:2000 dilution.
ARG57186 anti-Phosphothreonine antibody [RM102] IP image
Immunoprecipitation: Calyculin A / Okadaic Acid treated A431 cells by ARG57186 anti-Phosphothreonine antibody [RM102] at 1:500 dilution, and then blotted with the same antibody. 1) Whole cell lysate control, 2) IP by Rabbit IgG control, and 3) IP by ARG57186 anti-Phosphothreonine antibody [RM102].
ARG57186 anti-Phosphothreonine antibody [RM102] Specificity test image
ARG57186 anti-Phosphothreonine antibody [RM102] recognizes Phosphorylated threonine in peptides with different sequences. It has minimal cross-reactivity with phosphorylated serine.

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